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快速荧光抗体结合程序。

Rapid fluorescent-antibody conjugation procedure.

机构信息

U. S. Department of Health, Education, and Welfare, Public Health Service, Rocky Mountain Laboratory, National Institute of Allergy and Infectious Diseases, Hamilton, Montana 59840.

出版信息

Infect Immun. 1971 Feb;3(2):355-7. doi: 10.1128/iai.3.2.355-357.1971.

DOI:10.1128/iai.3.2.355-357.1971
PMID:16557977
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC416155/
Abstract

This note describes a technique developed for rapid preparation of specific and brilliant conjugates for direct staining of rickettsial agents in yolk-sac, tick, and vertebrate tissues.

摘要

本说明描述了一种技术，用于快速制备特定而鲜明的缀合物，以便直接对卵黄囊、蜱和脊椎动物组织中的立克次体进行染色。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f531/416155/549404710883/iai00278-0164-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f531/416155/fbc20ff16eee/iai00278-0164-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f531/416155/901b2a548ecd/iai00278-0164-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f531/416155/549404710883/iai00278-0164-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f531/416155/fbc20ff16eee/iai00278-0164-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f531/416155/901b2a548ecd/iai00278-0164-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f531/416155/549404710883/iai00278-0164-c.jpg

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Infect Immun. 1971 Feb;3(2):355-7. doi: 10.1128/iai.3.2.355-357.1971.
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本文引用的文献

1
Ultra-rapid fluorescent labelling of proteins.蛋白质的超快速荧光标记
Nature. 1962 Jan 13;193:167-8. doi: 10.1038/193167b0.
2
The use of fluorescent antibody in the diagnosis and study of viral and rickettsial infections.荧光抗体在病毒和立克次氏体感染的诊断及研究中的应用。
Ergeb Mikrobiol Immunitatsforsch Exp Ther. 1960;33:242-58. doi: 10.1007/978-3-662-42621-0_5.
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A PREPARATIVE METHOD FOR THE SEPARATION OF 7S GAMMA GLOBULIN FROM HUMAN SERUM.一种从人血清中分离7Sγ球蛋白的制备方法。
鉴定感染美国犬蜱（Dermacentor andersoni）的一种内共生菌为弗朗西斯菌属的成员。
Appl Environ Microbiol. 1997 Oct;63(10):3933-40. doi: 10.1128/aem.63.10.3933-3940.1997.
4
Ability of transovarially and subsequent transstadially infected Ixodes hexagonus ticks to maintain and transmit Borrelia burgdorferi in the laboratory.经卵巢感染并随后经不同发育阶段感染的六角硬蜱在实验室中维持和传播伯氏疏螺旋体的能力。
Exp Appl Acarol. 1993 Aug;17(8):581-6. doi: 10.1007/BF00053487.
5
Variable major proteins of Borrellia hermsii.赫氏疏螺旋体的可变主要蛋白
J Exp Med. 1982 Nov 1;156(5):1312-24. doi: 10.1084/jem.156.5.1312.
6
Antigenic variation of Borrelia hermsii.赫氏疏螺旋体的抗原变异
J Exp Med. 1982 Nov 1;156(5):1297-311. doi: 10.1084/jem.156.5.1297.
7
Biological and immunological properties of Coxiella burnetii vaccines in C57BL/10ScN endotoxin-nonresponder mice.伯氏考克斯氏体疫苗在C57BL/10ScN内毒素无反应小鼠中的生物学和免疫学特性
Infect Immun. 1982 Mar;35(3):1091-102. doi: 10.1128/iai.35.3.1091-1102.1982.
8
Biochemical stratagem for obligate parasitism of eukaryotic cells by Coxiella burnetii.伯氏考克斯氏体对真核细胞专性寄生的生化策略。
Proc Natl Acad Sci U S A. 1981 May;78(5):3240-4. doi: 10.1073/pnas.78.5.3240.
9
Serological evaluation of O fever in humans: enhanced phase I titers of immunoglobulins G and A are diagnostic for Q fever endocarditis.人类Q热的血清学评估：免疫球蛋白G和A的I期滴度升高对Q热心内膜炎具有诊断意义。
Infect Immun. 1983 Sep;41(3):1089-98. doi: 10.1128/iai.41.3.1089-1098.1983.
10
Q fever endocarditis.Q热心内膜炎
West J Med. 1984 Jun;140(6):943-5.
Arch Biochem Biophys. 1964 Dec;108:514-22. doi: 10.1016/0003-9861(64)90436-9.
4
FACTORS AFFECTING THE RATE OF REACTION OF FLUORESCEIN ISOTHIOCYANATE WITH SERUM PROTEINS.影响异硫氰酸荧光素与血清蛋白反应速率的因素
J Immunol. 1964 Aug;93:232-42.
5
The chromatographic purification of fluorescein-antibody.荧光素抗体的色谱纯化
J Histochem Cytochem. 1961 Sep;9:484-6. doi: 10.1177/9.5.484.
6
Fluorescent antibody methods.荧光抗体法
Gen Cytochem Methods. 1958;1:399-422.
7
Conjugation of fluorescein isothiocyanate to antibodies. I. Experiments on the conditions of conjugation.异硫氰酸荧光素与抗体的结合。I. 结合条件的实验。
Immunology. 1970 Jun;18(6):865-73.
8
Direct immunofluorescent tissue culture assay for lymphocytic choriomeningitis virus.淋巴细胞性脉络丛脑膜炎病毒的直接免疫荧光组织培养测定法
J Immunol. 1968 May;100(5):1135-8.
9
Conjugation of fluorescein isothiocyanate to antibodies. II. A reproducible method.异硫氰酸荧光素与抗体的结合。II. 一种可重复的方法。
Immunology. 1970 Jun;18(6):875-81.
10
A microagglutination technique for detection and measurement of rickettsial antibodies.一种用于检测和测量立克次氏体抗体的微量凝集技术。
Acta Virol. 1969 Jan;13(1):60-6.