通过流式细胞术估计单个淋巴细胞的膜电位。
Estimation of membrane potentials of individual lymphocytes by flow cytometry.
作者信息
Shapiro H M, Natale P J, Kamentsky L A
出版信息
Proc Natl Acad Sci U S A. 1979 Nov;76(11):5728-30. doi: 10.1073/pnas.76.11.5728.
Abstract
The membrane potentials of individual cells can be estimated by flow cytometric quantitation of the cells' uptake of the fluorescent lipophilic cationic dye 3,3'-dihexyloxacarbocyanine iodide. Human lymphocytes separated from peripheral blood on Hypaque-Ficoll gradients are uniformly depolarized by gramicidin and hyperpolarized by valinomycin. Concanavalin A and phytohemagglutinin depolarize only a fraction of the lymphocytes. The flow cytometric technique allows precise detection of heterogeneous membrane potential responses to stimuli such as lectins; it could also provide a basis for sorting cells that respond differently to a given stimulus.
摘要
单个细胞的膜电位可通过流式细胞术对细胞摄取荧光亲脂性阳离子染料碘化3,3'-二己基氧杂羰花青的定量来估计。在Hypaque-Ficoll梯度上从外周血中分离出的人淋巴细胞被短杆菌肽均匀去极化，被缬氨霉素超极化。伴刀豆球蛋白A和植物血凝素仅使一部分淋巴细胞去极化。流式细胞术技术能够精确检测淋巴细胞对诸如凝集素等刺激的异质性膜电位反应；它还可为分选对给定刺激有不同反应的细胞提供基础。
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