用酶联免疫吸附测定法（ELISA）和高效液相色谱法（HPLC）测定市售巴氏杀菌乳中黄曲霉毒素M1的含量。

Occurrence of aflatoxin M1 in commercial pasteurized milk determined with ELISA and HPLC.

作者信息

Markaki P, Melissari E

机构信息

Department of Food Chemistry, University of Athens, Greece.

出版信息

Food Addit Contam. 1997 Jul;14(5):451-6. doi: 10.1080/02652039709374551.

DOI:10.1080/02652039709374551

PMID:9328529

Abstract

Eighty-one samples of commercial pasteurized milk from Athens market were analysed for the presence of aflatoxin M1 (AFM1). A combination of a commercial ELISA kit and a modified HPLC method was applied for the rapid and reliable determination of AFM1. AFM1 concentrations in milk extracts were initially estimated by ELISA. Samples found to contain more than 5 ng/l were further quantitated by HPLC. Determination was performed after derivatization of AFM1 to its hydroxylated product AFM2a. The recovery of the HPLC method used was found to be close to 100%. Thirty-two samples contained aflatoxin M1 at levels of 2.5-5 ng/l, none contained more than 5 ng/l, while 31 contained only traces of aflatoxin (0.5-1 ng/l). In nine samples no AFM1 was detected. There was no seasonal influence on the aflatoxin content of the milk samples analysed.

摘要

对雅典市场上的81份市售巴氏杀菌乳样本进行了黄曲霉毒素M1（AFM1）检测。采用商用酶联免疫吸附测定（ELISA）试剂盒和改良高效液相色谱（HPLC）法相结合的方法，对AFM1进行快速可靠的测定。首先通过ELISA估计牛奶提取物中的AFM1浓度。发现含有超过5 ng/l的样本再通过HPLC进一步定量。在将AFM1衍生化为其羟基化产物AFM2a后进行测定。所使用的HPLC方法的回收率接近100%。32个样本中黄曲霉毒素M1含量为2.5 - 5 ng/l，没有样本含量超过5 ng/l，而31个样本仅含有微量黄曲霉毒素（0.5 - 1 ng/l）。9个样本中未检测到AFM1。所分析的牛奶样本中的黄曲霉毒素含量没有季节性影响。