Wiig M, Abrahamsson S O, Lundborg G
Department of Hand and Plastic Surgery, Uppsala University Hospital, Sweden.
J Hand Surg Am. 1997 Sep;22(5):818-25. doi: 10.1016/S0363-5023(97)80075-5.
One deep flexor tendon and its surrounding sheath of each hindpaw of 48 rabbits were transected and repaired in order to investigate the abilities of rabbit flexor tendons and synovial sheaths to synthesize DNA and matrix components during healing and to study the effects of hyaluronan (HA). After repair, HA or saline was injected between the tendon and the sheath. Short-term culture and labeling in vitro were used up to 6 weeks after surgery to determine synthesis of DNA, proteoglycan, collagen, and noncollagen protein. Within tendon repair sites, the rate of cell proliferation increased and reached a maximum 5 days after surgery; within repaired synovial sheaths, the rate immediately decreased. In the healing tendons, the rate of collagen synthesis decreased and the rate of noncollagen protein synthesis remained unchanged. The opposite results were found within the healing synovial sheaths. HA did not affect the rate of cell proliferation or matrix synthesis in healing tendons or surrounding sheaths. These results show that cellular activities differ between tendons and synovial sheaths during healing and that those activities may not be affected by HA.
为了研究兔屈肌腱和滑膜鞘在愈合过程中合成DNA和基质成分的能力以及透明质酸（HA）的作用，对48只兔每只后爪的一条屈肌腱及其周围腱鞘进行横断并修复。修复后，在肌腱和腱鞘之间注射HA或生理盐水。术后6周内采用短期体外培养和标记法来测定DNA、蛋白聚糖、胶原蛋白和非胶原蛋白的合成。在肌腱修复部位，细胞增殖速率增加，并在术后5天达到最大值；在修复的滑膜鞘内，该速率立即下降。在愈合的肌腱中，胶原蛋白合成速率下降，非胶原蛋白合成速率保持不变。在愈合的滑膜鞘内发现了相反的结果。HA不影响愈合肌腱或周围腱鞘的细胞增殖速率或基质合成。这些结果表明，愈合过程中肌腱和滑膜鞘的细胞活性不同，且这些活性可能不受HA的影响。
