Wiig M, Abrahamsson S O, Lundborg G
Department of Hand Surgery, Lund University, Malmö University Hospital, Sweden.
J Hand Surg Am. 1996 Jul;21(4):599-604. doi: 10.1016/S0363-5023(96)80010-4.
Hyaluronan (HA), a high-molecular-weight polysaccharide, has been suggested to play a possible role during the early stages of healing of a variety of connective tissues and when topically applied to decrease the formation of adhesions following tendon surgery. As the mechanisms of HA actions are still being discussed, this study was designed to assess the effects of HA on cell proliferation and synthesis of matrix components of deep flexor tendons in a well-defined culture system. Cell proliferation, measured as the radioactive 3H-thymidine uptake by cultured segments of rabbit flexor tendons, was inhibited by the addition of HA to the culture medium. HA of molecular weight 0.5 x 10(6) inhibited the uptake significantly at concentrations within the range of 0.1-2.0 mg/mL, HA of molecular weight 1.6 x 10(6) at 0.5-2.0 mg/mL, and HA of molecular weight 3.6 x 10(6) at 1.0-2.0 mg/mL, as compared to matched control groups. At the concentration of 2 mg/mL, HA of the molecular weights of 0.5, 0.8, 1.6, and 3.6 x 10(6) equally inhibited cell proliferation. No effect on synthesis of matrix components, measured as the radioactive incorporation of 35S-sulfate, 3H-hydroxyproline, and 3H-proline by cultured segments of rabbit flexor tendons, was observed. These findings show that exogenously applied HA may act as a modulator of flexor tendon fibroblast proliferation, indicating a possible mechanism for antiadhesive effects following administration after flexor tendon surgery.
透明质酸（HA）是一种高分子量多糖，有人认为它在多种结缔组织愈合的早期阶段可能发挥作用，并且局部应用时可减少肌腱手术后粘连的形成。由于HA作用的机制仍在讨论中，本研究旨在评估在明确的培养系统中HA对兔屈指深肌腱细胞增殖和基质成分合成的影响。通过培养的兔屈指肌腱节段对放射性3H-胸腺嘧啶核苷的摄取来测量细胞增殖，向培养基中添加HA可抑制细胞增殖。与匹配的对照组相比，分子量为0.5×10⁶的HA在0.1 - 2.0mg/mL浓度范围内显著抑制摄取，分子量为1.6×10⁶的HA在0.5 - 2.0mg/mL浓度下抑制摄取，分子量为3.6×10⁶的HA在1.0 - 2.0mg/mL浓度下抑制摄取。在2mg/mL浓度下，分子量为0.5、0.8、1.6和3.6×10⁶的HA对细胞增殖的抑制作用相同。未观察到对基质成分合成的影响，基质成分合成通过培养的兔屈指肌腱节段对35S-硫酸盐、3H-羟脯氨酸和3H-脯氨酸的放射性掺入来测量。这些发现表明，外源性应用的HA可能作为屈指肌腱成纤维细胞增殖的调节剂，这表明屈指肌腱手术后给药后产生抗粘连作用的一种可能机制。
