Mauer M M, Bartness T J
Department of Biology, Georgia State University, Atlanta 30303, USA.
Physiol Behav. 1997 Nov;62(5):1029-36. doi: 10.1016/s0031-9384(97)00233-3.
Long-day-housed Siberian hamsters show increases in the mass of nonexcised white adipose tissue (WAT) 12 weeks after bilateral removal (x) of epididymal WAT (EWAT). Although EWAT shows no significant regeneration, surviving EWAT appears to increase in mass to a small degree. The purpose of the present study was to determine the time course for fat pad mass and cellularity changes after EWATx and to test whether surviving EWAT adipocytes undergo hypertrophy or hyperplasia. Male Siberian hamsters underwent EWATx or sham EWATx (SHAM). At Week 0 and at 2-week intervals up to 12 weeks postsurgery, a representative sample of animals from each group were killed. EWAT, retroperitoneal WAT (RWAT), and inguinal WAT (IWAT) were removed, weighed, and processed for cellularity measurement. IWAT and, to a nonsignificant degree, RWAT, increased in mass after EWATx due to fat cell hypertrophy. These changes began as early as Week 4 postlipectomy, but no mass or cellularity change was significant until Week 12. The surviving EWAT adipocytes of EWATx hamsters also were larger than those of SHAM hamsters and, unlike EWAT adipocyte number, increased with time within the EWATx group. SHAM hamsters showed slight increases in the mass of EWAT, but not IWAT or RWAT, due to a nonsignificant doubling of EWAT adipocyte number during the 3 months of the experiment.
长期饲养的西伯利亚仓鼠在双侧切除附睾白色脂肪组织（EWAT）12周后，未切除的白色脂肪组织（WAT）质量增加。尽管EWAT没有明显的再生，但存活的EWAT质量似乎有小程度的增加。本研究的目的是确定EWAT切除后脂肪垫质量和细胞组成变化的时间进程，并测试存活的EWAT脂肪细胞是否发生肥大或增生。雄性西伯利亚仓鼠接受EWAT切除或假EWAT切除（假手术）。在术后0周以及术后2周为间隔直至12周，每组处死代表性的动物样本。取出附睾脂肪垫（EWAT）、腹膜后脂肪垫（RWAT）和腹股沟脂肪垫（IWAT），称重并进行细胞组成测量。EWAT切除后，IWAT以及在不显著程度上RWAT，由于脂肪细胞肥大质量增加。这些变化早在抽脂术后第4周就开始了，但直到第12周质量或细胞组成变化才显著。EWAT切除仓鼠的存活EWAT脂肪细胞也比假手术仓鼠的大，并且与EWAT脂肪细胞数量不同，在EWAT切除组中随时间增加。在实验的3个月期间，假手术仓鼠的EWAT质量略有增加，但IWAT和RWAT没有增加，这是由于EWAT脂肪细胞数量不显著地翻倍。
