Smith V, Chou K N, Lashkari D, Botstein D, Brown P O
Department of Biochemistry, Stanford University School of Medicine, Stanford, CA 94305, USA. Medicine, Stanford, CA 94305, USA.
Science. 1996 Dec 20;274(5295):2069-74. doi: 10.1126/science.274.5295.2069.
Genetic footprinting was used to assess the phenotypic effects of Ty1 transposon insertions in 268 predicted genes of chromosome V of Saccharomyces cerevisiae. When seven selection protocols were used, Ty1 insertions in more than half the genes tested (157 of 268) were found to result in a detectable reduction in fitness. Results could not be obtained for fewer than 3 percent of the genes tested (7 of 268). Previously known mutant phenotypes were confirmed, and, for about 30 percent of the genes, new mutant phenotypes were identified.
基因足迹法被用于评估酿酒酵母第五条染色体上268个预测基因中Ty1转座子插入的表型效应。当使用七种选择方案时，发现超过一半被测试基因（268个中的157个）中的Ty1插入导致可检测到的适应性降低。对于不到3%的被测试基因（268个中的7个）无法获得结果。先前已知的突变表型得到了证实，并且对于约30%的基因，鉴定出了新的突变表型。
