Groth I, Schumann P, Rainey F A, Martin K, Schuetze B, Augsten K
Hans-Knöll-Institut für Naturstoff-Forschung e.V., DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH, Aussenstelle Jena, Germany.
Int J Syst Bacteriol. 1997 Oct;47(4):1129-33. doi: 10.1099/00207713-47-4-1129.
A novel actinomycete was isolated from compost soil and was studied taxonomically and phylogenetically. Cells of this organism were gram positive, not acid fast, nonmotile, nonsporulating, irregular coccoid to short rod shaped, and microaerophilic. The cell wall peptidoglycan contained lysine and was cross-linked via an L-Lys<--L-Ser<--D-Asp interpeptide bridge. The major menaquinone was MK-8(H4). The polar lipids were phosphatidylinositol, phosphatidylglycerol, diphosphatidylglycerol, phosphatidylethanolamine, and two unknown phospholipids. Mycolic acids were absent. The cellular fatty acid profile was complex, with large amounts of saturated and monounsaturated straight-chain acids and smaller amounts of iso and anteiso branched-chain acids. The G+C content of the DNA was 66 mol%. Comparative 16S ribosomal DNA studies revealed that strain HKI 0089T represents a novel lineage within Actinobacteria (32) distinct from all previously described genera and most closely related to members of the genera Kytococcus, Dermacoccus, and Dermatophilus of the family Dermatophilaceae. On the basis of our results, we suggest that strain HKI 0089 should be classified in a new genus and species, for which we propose the name Demetria terragena. The type strain and the only strain of the genus and species is HKI 0089 (DSM 11295).
从堆肥土壤中分离出一种新型放线菌，并对其进行了分类学和系统发育学研究。该微生物的细胞革兰氏阳性，抗酸染色阴性，不运动，不产芽孢，呈不规则球状至短杆状，微需氧。细胞壁肽聚糖含有赖氨酸，通过L-Lys<--L-Ser<--D-Asp肽间桥交联。主要的甲基萘醌是MK-8(H4)。极性脂质为磷脂酰肌醇、磷脂酰甘油、二磷脂酰甘油、磷脂酰乙醇胺和两种未知的磷脂。不存在分枝菌酸。细胞脂肪酸谱复杂，含有大量饱和和单不饱和直链酸以及少量异链和anteiso支链酸。DNA的G+C含量为66 mol%。比较16S核糖体DNA研究表明，菌株HKI 0089T代表放线菌中的一个新谱系(32)，与所有先前描述的属不同，与嗜皮菌科的Kytococcus属、Dermacoccus属和嗜皮菌属的成员关系最密切。根据我们的结果，我们建议将菌株HKI 0089分类为一个新的属和种，我们为此提议命名为土栖德美菌（Demetria terragena）。该属和种的模式菌株和唯一菌株是HKI 0089(DSM 11295)。
