Groth I, Schumann P, Schuetze B, Augsten K, Kramer I, Stackebrandt E
Hans-Knöll-Institut für Naturstoff-Forschung e.V., Jena, Germany.
Int J Syst Bacteriol. 1999 Oct;49 Pt 4:1733-40. doi: 10.1099/00207713-49-4-1733.
Two aerobic, Gram-positive bacteria, strains HKI 0122T and HKI 0132, were isolated from a cave. Cells are not acid-fast, non-motile, non-spore-forming and exhibit a rod-coccus growth cycle. The cell wall peptidoglycan contains lysine in position 3 of the peptide subunit and an interpeptide bridge of L-Lys<--L-Glu. The major menaquinone is MK-8(H4), 13-methyl and 12-methyl tetradecanoic acids are the predominating fatty acids. The polar lipids consist of phosphatidylinositol, diphosphatidylglycerol and three unknown phospholipids. Mycolic acids are absent. The DNA base composition is 71 mol% G + C. Phylogenetic analysis revealed that strain HKI 0122T forms a novel taxon among the families and unassigned genera of the suborder Micrococcineae, within the order Actinomycetales. On the basis of the genotypic, chemotaxonomic, morphological and physiological characteristics of these two isolates it is proposed to assign strains HKI 0122T and HKI 0132 to a new genus and species for which the name Beutenbergia cavernae gen. nov., sp. nov. is proposed. The type strain is HKI 0122T (= DSM 12333T).
从一个洞穴中分离出两株需氧革兰氏阳性菌，菌株HKI 0122T和HKI 0132。细胞抗酸染色阴性、无运动性、不形成芽孢，呈现杆状-球菌生长周期。细胞壁肽聚糖在肽亚基的第3位含有赖氨酸，肽间桥为L-Lys<--L-Glu。主要的甲基萘醌是MK-8(H4)，13-甲基和12-甲基十四烷酸是主要脂肪酸。极性脂质由磷脂酰肌醇、二磷脂酰甘油和三种未知磷脂组成。不存在分枝菌酸。DNA的碱基组成为71 mol% G + C。系统发育分析表明，菌株HKI 0122T在放线菌目微球菌亚目的科和未分类属中形成一个新的分类单元。基于这两个分离株的基因型、化学分类学、形态学和生理学特征，建议将菌株HKI 0122T和HKI 0132归为一个新属新种，命名为洞穴贝氏菌（Beutenbergia cavernae），模式菌株为HKI 0122T (= DSM 12333T)。
