Gniadecka M, Wulf H C, Nielsen O F, Christensen D H, Hercogova J
Department of Dermatology, Bispebjerg Hospital, University of Copenhagen, Denmark.
Photochem Photobiol. 1997 Oct;66(4):418-23. doi: 10.1111/j.1751-1097.1997.tb03167.x.
Near-infrared Fourier transform Raman spectroscopy is an analytical, nondestructive technique that provides information about the molecular structure of the investigated sample. The molecular structure of proteins and lipids differ between neoplastic and normal tissues and therefore Raman spectroscopy has been considered promising for the diagnosis of cancer. We aimed to compare the molecular structure of normal skin, benign and malignant skin lesions by the near-infrared Fourier transform Raman spectroscopy. Biopsies were obtained from the following skin lesions: skin tag, dermatofibroma, seborrhoeic keratosis, actinic keratosis, keratoacanthoma, basal cell carcinoma, squamous cell carcinoma, nevus intradermalis, nevus compositus, dysplastic nevus and lentigo maligna. Control skin was harvested from the vicinity of these lesions. In the Raman spectra, the secondary structure of the proteins was reflected by the amide vibrations of peptide bonds. The principal lipid vibrations were twisting and wagging (CH2) and CH stretching vibrations. Histologically distinguishable lesions showed specific combinations of band changes indicating alterations in the protein conformation and in the molecular structure of the lipids. Histogenetically related lesions (actinic keratosis and sqamous cell carcinoma) produced similar but not identical patterns of spectral changes. Because the examined skin lesions produced reproducible and unique spectra, we suggest that Raman spectroscopy will be useful for diagnosis of skin lesions.
近红外傅里叶变换拉曼光谱法是一种分析性的非破坏性技术，可提供有关被研究样品分子结构的信息。肿瘤组织和正常组织中蛋白质和脂质的分子结构不同，因此拉曼光谱法被认为在癌症诊断方面具有前景。我们旨在通过近红外傅里叶变换拉曼光谱法比较正常皮肤、良性和恶性皮肤病变的分子结构。从以下皮肤病变获取活检样本：皮赘、皮肤纤维瘤、脂溢性角化病、光化性角化病、角化棘皮瘤、基底细胞癌、鳞状细胞癌、皮内痣、混合痣、发育异常痣和恶性雀斑。对照皮肤取自这些病变附近。在拉曼光谱中，蛋白质的二级结构由肽键的酰胺振动反映。主要的脂质振动是扭曲和摇摆（CH2）以及CH伸缩振动。组织学上可区分的病变显示出特定的谱带变化组合，表明蛋白质构象和脂质分子结构发生了改变。组织发生学相关的病变（光化性角化病和鳞状细胞癌）产生了相似但不完全相同的光谱变化模式。由于所检查的皮肤病变产生了可重复且独特的光谱，我们认为拉曼光谱法将有助于皮肤病变的诊断。
