Sancho E, Ferrando M D, Andreu E
Dept. Animal Physiology, Faculty of Biological Sciences, Burjasot, Valencia, Spain.
J Environ Sci Health B. 1997 Nov;32(6):915-28. doi: 10.1080/03601239709373120.
European eel (Anguilla anguilla) were exposed to sublethal fenitrothion concentrations in a continuous flow-through system for 4 days. Muscle acetylcholinesterase (AChE) activity was evaluated after 2, 8, 12, 24, 32, 48, 56, 72 and 96 hours pesticide exposure. Results showed that AChE activity in eel muscle tissue decreased as concentration of fenitrothion increased. Pesticide induced significant inhibitory effects on the AChE activity of A. anguilla ranging from > 35% inhibition at sublethal concentration of 0.02 ppm to > 44% inhibition at sublethal concentration of 0.04 ppm. Eel were exposed to both fenitrothion concentrations for 96 hours and then allowed a period of recovery in pesticide-free water. Samples were taken out at 8, 12, 24, 48, 72, 96, 144 and 192 hours and eel muscle AChE activity was evaluated. Following 1 week of recovery, the AChE activity for those animals previously exposed to 0.02 and 0.04 ppm fenitrothion was still different from the controls. So, the AChE activity of eel muscle at the end of the recovery phases remained significantly depressed.
将欧洲鳗鲡（Anguilla anguilla）置于连续流动系统中，暴露于亚致死浓度的杀螟硫磷中4天。在暴露于农药2、8、12、24、32、48、56、72和96小时后，评估肌肉乙酰胆碱酯酶（AChE）活性。结果表明，鳗鲡肌肉组织中的AChE活性随杀螟硫磷浓度的增加而降低。农药对欧洲鳗鲡的AChE活性产生了显著的抑制作用，亚致死浓度为0.02 ppm时抑制率> 35%，亚致死浓度为0.04 ppm时抑制率> 44%。将鳗鲡暴露于这两种杀螟硫磷浓度下96小时，然后在无农药的水中恢复一段时间。在8、12、24、48、72、96、144和192小时取出样本，评估鳗鲡肌肉AChE活性。恢复1周后，那些先前暴露于0.02和0.04 ppm杀螟硫磷的动物的AChE活性仍与对照组不同。因此，恢复阶段结束时鳗鲡肌肉的AChE活性仍显著降低。
