Sancho E, Ferrando M D, Andreu E
Department of Animal Physiology, Faculty of Biological Sciences, Dr. Moliner 50, Burjasot, Valencia, 46100, Spain.
Ecotoxicol Environ Saf. 1997 Nov;38(2):132-6. doi: 10.1006/eesa.1997.1573.
European eels (Anguilla anguilla) were exposed to sublethal fenitrothion concentrations (0.02 and 0.04 mg/liter) in a continuous flow-through system for 4 days. Gill Mg2+- and Na+,K+-ATPase activities were evaluated after 2, 8, 12, 24, 32, 48, 56, 72, and 96 h of pesticide exposure. Results indicated that ATPase activity in gill tissue decreased as concentration of fenitrothion increased. Pesticide induced significant inhibitory effects on the Na+, K+-ATPase activity of A. anguilla, ranging from >56% inhibition at a sublethal concentration of 0.02 ppm to >73% inhibition at a sublethal concentration of 0.04 ppm. Eels were exposed to both fenitrothion concentrations for 96 h and then allowed a period of recovery in pesticide-free water. Samples were removed at 8, 12, 24, 48, 72, 96, 144, and 192 h and eel gill ATPase activity was evaluated. Following 1 week of recovery, the Na+,K+-ATPase activity for those animals previously exposed to 0.04 ppm fenitrothion was still different from that of the controls.
在连续流动系统中，将欧洲鳗鲡（Anguilla anguilla）暴露于亚致死浓度的杀螟硫磷（0.02和0.04毫克/升）中4天。在接触农药2、8、12、24、32、48、56、72和96小时后，评估鳃中Mg2 + -和Na +，K + -ATP酶的活性。结果表明，鳃组织中的ATP酶活性随着杀螟硫磷浓度的增加而降低。农药对欧洲鳗鲡的Na +，K + -ATP酶活性产生了显著的抑制作用，在亚致死浓度为0.02 ppm时抑制率大于56％，在亚致死浓度为0.04 ppm时抑制率大于73％。将鳗鲡暴露于两种杀螟硫磷浓度下96小时，然后在无农药的水中恢复一段时间。在8、12、24、48、72、96、144和192小时取样，评估鳗鲡鳃ATP酶活性。恢复1周后，先前暴露于0.04 ppm杀螟硫磷的那些动物的Na +，K + -ATP酶活性仍与对照组不同。
