Sancho E, Ferrando M D, Andreu E
Department of Animal Physiology, Faculty of Biological Sciences, Valencia, Spain.
Ecotoxicol Environ Saf. 1997 Dec;38(3):205-9. doi: 10.1006/eesa.1997.1579.
European eel (Anguilla anguilla) were exposed to sublethal fenitrothion concentrations in a continuous flow-through system for 4 days. Brain acetylcholinesterase (AChE) activity was evaluated after 2, 8, 12, 24, 32, 48, 56, 72, and 96 h pesticide exposure. Results indicated that AChE activity in eel brains decreased as the concentration of fenitrothion increased. The pesticide induced significant inhibitory effects on the AchE activity of A. anguilla, ranging from > 40% inhibition at a sublethal concentration of 0.02 ppm to > 60% inhibition at a sublethal concentration of 0.04 ppm. Eel were exposed to both fenitrothion concentrations for 96 h and then allowed a period of recovery in pesticide-free water. Samples were removed at 8, 12, 24, 48, 72, 96, 144, and 192 h and eel brain AChE activity was evaluated. Following 1 week of recovery, the AChE activity of those animals previously exposed to 0.02 and 0.04 ppm fenitrothion was still different from that of the controls. So, the AChE activity of eel brains at the end of the recovery phase remained significantly depressed.
将欧洲鳗鲡（Anguilla anguilla）置于连续流动通过系统中，暴露于亚致死浓度的杀螟硫磷中4天。在暴露于农药2、8、12、24、32、48、56、72和96小时后，评估其脑乙酰胆碱酯酶（AChE）活性。结果表明，鳗鲡脑中的AChE活性随杀螟硫磷浓度的增加而降低。该农药对欧洲鳗鲡的AChE活性产生了显著的抑制作用，在0.02 ppm的亚致死浓度下抑制率> 40%，在0.04 ppm的亚致死浓度下抑制率> 60%。将鳗鲡暴露于这两种杀螟硫磷浓度下96小时，然后在无农药的水中让其恢复一段时间。在8、12、24、48、72、96、144和192小时取样，评估鳗鲡脑AChE活性。恢复1周后，之前暴露于0.02和0.04 ppm杀螟硫磷的动物的AChE活性仍与对照组不同。因此，恢复阶段结束时鳗鲡脑的AChE活性仍显著降低。
