Fernández-Vega C, Sancho E, Ferrando M D, Andreu-Moliner E
Dept. Animal Physiology, Faculty of Biology, University of Valencia, Spain.
J Environ Sci Health B. 1999 Jan;34(1):61-73. doi: 10.1080/03601239909373184.
The acute toxicity of the herbicide thiobencarb (S-4-chlorobenzyl diethylthiocarbamate) was determined for the European eel (Anguilla anguilla). The 24, 48, 72 and 96 hours median lethal concentrations (LC50) were 25.7, 21.7, 17.0 and 13.2 mg/L, respectively. Fish were also exposed to a sublethal thiobencarb concentration (1/60 LC50-96 hr = 0.22 mg/L) during 96 hours in a flow-through system and then an elimination period of 192 hours in clean water was allowed. Eels were removed and blood samples taken out at each exposure time and recovery period in order to evaluate AChE activity. Thiobencarb induced significant inhibitory effects on plasma AChE activity of A. anguilla from the first contact time with the toxicant. This inhibition (under 50% activity) was maintained during the entire exposure period (96 hours) and even those animals transferred to clean water showed plasma AChE activity different from the controls. Differences between total and specific AChE activity were detected during the exposure period. Total AChE activity in the plasma from animals transferred to a medium free of toxicant recovered its normal value while specific AChE activity remained depressed (< 50%) until five days later.
测定了除草剂杀草丹（S-4-氯苄基二乙基硫代氨基甲酸盐）对欧洲鳗鲡（Anguilla anguilla）的急性毒性。24、48、72和96小时的半数致死浓度（LC50）分别为25.7、21.7、17.0和13.2毫克/升。在流通系统中，鱼还暴露于亚致死浓度的杀草丹（1/60 LC50-96小时 = 0.22毫克/升）96小时，然后在清洁水中进行192小时的消除期。在每次暴露时间和恢复期取出鳗鱼并采集血样，以评估乙酰胆碱酯酶（AChE）活性。从与毒物首次接触时起，杀草丹就对欧洲鳗鲡的血浆AChE活性产生了显著的抑制作用。这种抑制（活性低于50%）在整个暴露期（96小时）内持续存在，甚至那些转移到清洁水中的动物的血浆AChE活性也与对照组不同。在暴露期检测到总AChE活性和特异性AChE活性之间的差异。转移到无毒培养基中的动物血浆中的总AChE活性恢复到正常值，而特异性AChE活性在五天后仍保持在较低水平（<50%）。
