Markx G H, Dyda P A, Pethig R
Institute of Molecular and Biomolecular Electronics, University of Wales, Bangor, Gwynedd, UK.
J Biotechnol. 1996 Nov 1;51(2):175-80. doi: 10.1016/0168-1656(96)01617-3.
Dielectrophoresis, the lateral motion induced on particles by non-uniform electric fields, is a sensitive function of the electrical conductivity of the particle suspending medium. This dependence is exploited in a new technique for separating bioparticles from suspended mixtures. The bioparticles are first immobilised by positive dielectrophoresis at electrodes in a separation chamber, and the conductivity of the liquid flowing through the chamber is then gradually and continuously increased so as to produce a conductivity gradient with time. The bioparticles are released from the electrodes according to their own dielectric properties and as a function of flow rate and medium conductivity. This is demonstrated for pure suspensions and mixtures of the bacteria Bacillus subtilis, Escherichia coli and Micrococcus luteus.
介电泳是指非均匀电场在粒子上引起的横向运动，它是粒子悬浮介质电导率的敏感函数。这种依赖性被应用于一种从悬浮混合物中分离生物粒子的新技术中。首先通过正介电泳将生物粒子固定在分离室中的电极上，然后逐渐连续地增加流过该室的液体的电导率，以便随时间产生电导率梯度。生物粒子根据其自身的介电特性以及流速和介质电导率从电极上释放出来。这在枯草芽孢杆菌、大肠杆菌和藤黄微球菌的纯悬浮液和混合物中得到了证明。
