Fontana C, Laratta E, Marino D, Pistoia E S, Favalli C
Department of Experimental Medicine and Biochemical Science, University of Rome Tor Vergata, Italy.
Eur J Clin Microbiol Infect Dis. 1997 Sep;16(9):689-92. doi: 10.1007/BF01708562.
In order to correctly identify a new biovar of Staphylococcus aureus subsp, aureus, (NBSA) a simple, rapid, and reliable enzymatic assay was developed. The assay was based on the detection of the production of three enzymes: alpha-glucosidase, beta-glucosidase and beta-N-acetyl-glucosaminidase. Of a total of 46 isolates of Staphylococcus aureus subsp. aureus from clinical specimens, the new assay correctly identified 19 as NBSA and 27 as typical Staphylococcus aureus subsp. aureus. Among the 19 NBSA isolates, 15 (79%) showed a clear biochemical profile while only four isolates (21%) showed a less well-defined enzymatic combination, due to a phenotypic alteration caused by subculturing. Since this assay is both simple to perform and inexpensive, it is potentially applicable in the laboratory.
为了正确鉴定金黄色葡萄球菌亚种（NBSA）的一种新生物变种，开发了一种简单、快速且可靠的酶促检测方法。该检测方法基于对三种酶的产生进行检测：α-葡萄糖苷酶、β-葡萄糖苷酶和β-N-乙酰葡萄糖胺酶。在从临床标本中分离出的总共46株金黄色葡萄球菌亚种金黄色葡萄球菌中，新检测方法正确鉴定出19株为NBSA，27株为典型的金黄色葡萄球菌亚种金黄色葡萄球菌。在19株NBSA分离株中，15株（79%）呈现出清晰的生化特征，而只有4株分离株（21%）由于传代培养导致的表型改变，呈现出不太明确的酶组合。由于该检测方法操作简单且成本低廉，因此在实验室中具有潜在的应用价值。
