Eggert C
Institut für Allgemeine Mikrobiologie und Mikrobengenetik, Friedrich-Schiller Universität Jena, Germany.
Microbiol Res. 1997 Sep;152(3):315-8. doi: 10.1016/S0944-5013(97)80046-8.
Concentrated culture fluid of the wood-rotting basidiomycete Pycnoporus cinnabarinus showed biological activity against a variety of bacterial strains. The maximal inhibitory effect was obtained for Gram-positive bacteria of the genus Streptococcus. In general, inhibition was higher for Gram-positive than Gram-negative bacteria. P. cinnabarinus produces the phenoxazinone derivative, cinnabarinic acid. This red pigment accumulates in sporocarps as well as in liquid cultures. As shown previously, laccase secreted by the fungus oxidizes the precursor 3-hydroxyanthranilic acid to cinnabarinic acid. The present study demonstrates that this reaction is necessary for the production of antibacterial compounds by the fungus. The biological activity of concentrated P. cinnabarinus culture fluid was nearly identical with that of cinnabarinic acid, synthesized by purified laccase in vitro.
木腐担子菌朱红密孔菌的浓缩培养液对多种细菌菌株显示出生物活性。对链球菌属革兰氏阳性菌的抑制作用最大。一般来说，革兰氏阳性菌的抑制作用高于革兰氏阴性菌。朱红密孔菌产生苯并恶嗪酮衍生物——朱红菌素。这种红色色素在子实体以及液体培养物中积累。如先前所示，该真菌分泌的漆酶将前体3-羟基邻氨基苯甲酸氧化为朱红菌素。本研究表明，该反应是该真菌产生抗菌化合物所必需的。朱红密孔菌浓缩培养液的生物活性与体外纯化漆酶合成的朱红菌素的生物活性几乎相同。
