幼鼠低接种量肺炎链球菌肺内感染模型的建立
Development of a model of low-inoculum Streptococcus pneumoniae intrapulmonary infection in infant rats.
作者信息
Saladino R A, Stack A M, Fleisher G R, Thompson C M, Briles D E, Kobzik L, Siber G R
机构信息
Department of Medicine, Children's Hospital, Boston, Massachusetts 02115, USA.
出版信息
Infect Immun. 1997 Nov;65(11):4701-4. doi: 10.1128/iai.65.11.4701-4704.1997.
Abstract
We have developed a model of low-inoculum Streptococcus pneumoniae infection in infant rats. We challenged 4-day-old Sprague-Dawley pups via intraperitoneal or intrapulmonary injection of S. pneumoniae serotypes 1, 3, 4, 5, 6b, 7f, 9v, 14, 19f, and 23f. To achieve bacteremia with low inocula, it was necessary to passage the isolates in rats. Inocula of the 10 S. pneumoniae serotypes producing bacteremia in 50% or more animals ranged from 1 to 400 CFU. Virulence was similar by intraperitoneal and intrapulmonary routes. Lung specimens from animals challenged by the intrapulmonary route grew S. pneumoniae and demonstrated histologic evidence of focal infection. Meningitis was detected in 20 to 50% of bacteremic animals, and mortality invariably followed bacteremia within 24 to 48 h. This model of intrapulmonary infection uses low inocula of S. pneumoniae and results in bacteremia, meningitis, and death in infant rats.
摘要
我们建立了幼鼠低接种量肺炎链球菌感染模型。通过腹腔内或肺内注射肺炎链球菌1、3、4、5、6b、7f、9v、14、19f和23f血清型，对4日龄的斯普拉格-道利幼鼠进行攻毒。为了用低接种量实现菌血症，有必要在大鼠体内传代分离株。在50%或更多动物中产生菌血症的10种肺炎链球菌血清型的接种量范围为1至400 CFU。腹腔内和肺内途径的毒力相似。经肺内途径攻毒的动物的肺标本培养出肺炎链球菌，并显示有局灶性感染的组织学证据。在20%至50%的菌血症动物中检测到脑膜炎，菌血症后24至48小时内 invariably 会死亡。这种肺内感染模型使用低接种量的肺炎链球菌，可导致幼鼠菌血症、脑膜炎和死亡。
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