Paxson J A, Weber J G, Kulczycki A
Department of Medicine, Washington University School of Medicine, St. Louis, Missouri 63110, USA.
Diabetes. 1997 Nov;46(11):1711-7. doi: 10.2337/diab.46.11.1711.
Early dietary exposure to cow's milk proteins has been proposed as an important environmental factor in the development of IDDM both in humans and in diabetes-prone rodents. To examine the significance of cow's milk protein in IDDM, 120 NOD mice were maintained, starting from conception until sacrifice, on one of four diets: standard PMI Picolab Rodent Diet 20, a milk-free modification of the standard Picolab diet, a milk-free diet incorporating 0.036% bovine serum albumin (BSA), and a milk-free diet including 0.036% bovine IgG (BGG). The cumulative IDDM incidence at 7 months for these mice in a specific pathogen-free environment on the respective diets was 78, 93, 93, and 67% for females, and 17, 54, 17, and 0% for males. The ages of diabetes onset and insulitis scores were similar for mice on each diet. The unexpectedly lower incidence of IDDM in mice on the milk-free diet that included BGG raises the possibility this cow's milk protein might possibly have some protective effect against the development of IDDM in NOD mice. Our main finding was that the standard, milk-free, and BSA-containing diets resulted in comparable incidences of IDDM in NOD mice, demonstrating that neither cow's milk whey proteins in general nor BSA in particular are significantly important as etiologic dietary agents in IDDM in NOD mice.
早期接触牛奶蛋白已被认为是人类和易患糖尿病的啮齿动物患胰岛素依赖型糖尿病（IDDM）的一个重要环境因素。为了研究牛奶蛋白在IDDM中的重要性，从受孕开始直至处死，120只非肥胖型糖尿病（NOD）小鼠被分别饲养在四种饮食中的一种上：标准的PMI Picolab啮齿动物饮食20号、标准Picolab饮食的无奶改良版、含有0.036%牛血清白蛋白（BSA）的无奶饮食以及含有0.036%牛免疫球蛋白G（BGG）的无奶饮食。在特定无病原体环境下，这些小鼠在各自饮食条件下7个月时的IDDM累积发病率，雌性分别为78%、93%、93%和67%，雄性分别为17%、54%、17%和0%。每种饮食喂养的小鼠的糖尿病发病年龄和胰岛炎评分相似。在含有BGG的无奶饮食喂养的小鼠中，IDDM发病率意外较低，这增加了这种牛奶蛋白可能对NOD小鼠IDDM的发展具有某种保护作用的可能性。我们的主要发现是，标准饮食、无奶饮食和含BSA的饮食在NOD小鼠中导致的IDDM发病率相当，这表明一般而言牛奶乳清蛋白，特别是BSA，在NOD小鼠IDDM中作为病因性饮食因素并不具有显著重要性。
