Matheny J L, Northup C M, Hofman W F
Res Commun Chem Pathol Pharmacol. 1976 Mar;13(3):411-23.
Electrically driven rabbit left atria were exposed to 20 min periods of either hypoxia or anoxia in the presence of a bicarbonate-phosphate buffer, a Tris buffer [tris (hydroxymethyl) aminomethane hydrochloride] or combination of both. The bicarbonate-phosphate buffer system was shown to be important for tissue survival during hypoxia or anoxia whereas recovery was diminished in the presence of Tris only. Stimulus threshold and arrhythmias were shown to increase for atria in Tris. Oxygen consumption determinations on both spontaneously beating right atria and quiescent left atria showed no difference between pre- and post-hypoxia or between different buffers. Tris was shown to elicit a positive inotropic effect without an increase in O2 consumption.
在碳酸氢盐 - 磷酸盐缓冲液、Tris缓冲液[三（羟甲基）氨基甲烷盐酸盐]或两者组合存在的情况下，将电驱动的兔左心房暴露于20分钟的缺氧或无氧环境中。结果表明，碳酸氢盐 - 磷酸盐缓冲系统对于缺氧或无氧期间的组织存活很重要，而仅在Tris存在的情况下恢复能力会降低。研究显示，Tris中的心房刺激阈值和心律失常会增加。对自发跳动的右心房和静止的左心房进行的耗氧量测定表明，缺氧前后或不同缓冲液之间没有差异。结果表明，Tris可引发正性肌力作用，而不会增加耗氧量。
