含2,6 - 二氨基嘌呤的肽核酸寡聚物的DNA结合及序列识别能力增强。
Increased DNA binding and sequence discrimination of PNA oligomers containing 2,6-diaminopurine.
作者信息
Haaima G, Hansen H F, Christensen L, Dahl O, Nielsen P E
机构信息
Center for Biomolecular Recognition, Department of Chemistry, The H. C.Orsted Institute, Universitetsparken 5, DK-2100 Copenhagen O, Denmark.
出版信息
Nucleic Acids Res. 1997 Nov 15;25(22):4639-43. doi: 10.1093/nar/25.22.4639.
Abstract
The synthesis of a diaminopurine PNA monomer, N-[N6-(benzyloxycarbonyl)-2,6-diaminopurine-9-yl] acetyl-N-(2-t-butyloxycarbonylaminoethyl)glycine, and the incorporation of this monomer into PNA oligomers are described. Substitution of adenine by diaminopurine in PNA oligomers increased the T m of duplexes formed with complementary DNA, RNA or PNA by 2.5-6.5 degrees C per diaminopurine. Furthermore, discrimination against mismatches facing the diaminopurine in the hybridizing oligomer is improved. Finally, a homopurine decamer PNA containing six diaminopurines is shown to form a (gel shift) stable strand displacement complex with a target in a 246 bp double-stranded DNA fragment.
摘要
描述了二氨基嘌呤PNA单体N-[N6-(苄氧羰基)-2,6-二氨基嘌呤-9-基]乙酰基-N-(2-叔丁氧羰基氨基乙基)甘氨酸的合成，以及该单体掺入PNA寡聚物的过程。在PNA寡聚物中用二氨基嘌呤取代腺嘌呤，使得与互补DNA、RNA或PNA形成的双链体的熔解温度（Tm）每增加一个二氨基嘌呤就升高2.5-6.5摄氏度。此外，杂交寡聚物中面对二氨基嘌呤的错配识别能力得到了提高。最后，含有六个二氨基嘌呤的同型嘌呤十聚体PNA与246bp双链DNA片段中的靶标形成了（凝胶迁移）稳定的链置换复合物。
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