Rodriguez N, De Lima H, Rodriguez A, Brewster S, Barker D C
Molteno Laboratories, Department of Pathology, University of Cambridge, UK.
Parasitology. 1997 Oct;115 ( Pt 4):349-58. doi: 10.1017/s003118209700139x.
In this paper the Leishmania (Viannia) braziliensis complex is defined as containing all species of the actual subgenus Viannia. Organisms of the L. (V) braziliensis complex are the causative agents of localized human cutaneous and mucocutaneous leishmaniasis in South America, much of Central America and some ares of North America. In our search for better species and subspecies diagnostic probes we focused our research on repetitive DNA, since it provides a greater number of target sites for hybridization. In this work we report the isolation and sequencing of a 1.8 kb DNA region, LbJ38, which is probably tandemly repeated or dispersed at least 4 times along one chromosome and is naturally present in L. (V) braziliensis genomic DNA. This region contains microsatellites and simple repeat DNA sequences and was isolated by screening a genomic DNA cosmid library with complex- and species-specific probes. No homology was found with other Leishmania microsatellite or repetitive DNA. The utility of this repetitive sequence and primers derived from it in the identification of L. (V) braziliensis is demonstrated. As far as we are aware, this is the first report of sequence characterized repetitive microsatellite and GC rich simple repeat DNA from the nuclear genome of New World Leishmania.
在本文中，巴西利什曼原虫（维阿尼亚种）复合体被定义为包含当前维阿尼亚种的所有物种。巴西利什曼原虫（维阿尼亚种）复合体的生物体是南美洲、中美洲大部分地区和北美洲一些地区人类局部皮肤和黏膜皮肤利什曼病的病原体。在我们寻找更好的物种和亚种诊断探针的过程中，我们将研究重点放在了重复DNA上，因为它为杂交提供了更多的靶位点。在这项工作中，我们报告了一个1.8 kb DNA区域LbJ38的分离和测序，该区域可能沿一条染色体串联重复或分散至少4次，并且天然存在于巴西利什曼原虫（维阿尼亚种）的基因组DNA中。该区域包含微卫星和简单重复DNA序列，是通过用复合体特异性和物种特异性探针筛选基因组DNA黏粒文库而分离得到的。未发现与其他利什曼原虫微卫星或重复DNA有同源性。证明了该重复序列及其衍生引物在鉴定巴西利什曼原虫（维阿尼亚种）中的实用性。据我们所知，这是关于来自新大陆利什曼原虫核基因组的序列特征性重复微卫星和富含GC的简单重复DNA的首次报道。
