Perl W, Silverman F, Delea A C, Chinard F P
Am J Physiol. 1976 Jun;230(6):1708-21. doi: 10.1152/ajplegacy.1976.230.6.1708.
Bolus injection of T-1824-albumin, test indicator, and tritiated water into a jugular vein of the anesthetized dog and sequential sampling of blood from a carotid artery yielded multiple-indicator outflow patterns for the lung. Permeability-surface products of the test indicator for the lung endothelial barrier were obtained by comparison of test indicator with T-1824-albumin on the upslope of the test-indicator curve and correction for backdiffusion. The derived endothelial permeability coefficients, based on surface area/wet lung weight-500 cm2/g (mean +/- 2 SE, 10(-5) cm s(-1)), were: sodium ion, 2.9 +/- 0.8; ethylene glycol, 7.3 +/- 1.5; 1, 3-propranediol, 7.9 +/- 3.2; 1, 2-propanediol, 10 +/- 4; 1, 4-butanediol, 14 +/- 8; 1, 5-pentanediol, 21 +/- 6; 1, 6-hexanediol, 41 +/- 11; formamide, 16 +/- 9; acetamide, 13 +/- 4; propionamide, 31 +/- 12; butyramide, 42 +/- 24; valeramide, 79 +/- 12; tritiated water, 150 +/- 50. The backdiffusion correction varies from 8% for sodium to 75% for valeramide. A parallel-pathway model of blood-tissue passive exchange of small nonelectrolyte solutes is compatible with these results, with a lipid pathway through endothelial cells and an aqueous pathway possibly through interendothelial clefts.
将测试指示剂T-1824白蛋白和氚化水注入麻醉犬的颈静脉，并从颈动脉顺序采集血液样本，得到了肺部的多指示剂流出模式。通过在测试指示剂曲线的上升段将测试指示剂与T-1824白蛋白进行比较，并对反向扩散进行校正，得出了肺内皮屏障的测试指示剂的渗透表面积乘积。基于表面积/湿肺重量为500 cm²/g（平均值±2标准误，10⁻⁵ cm s⁻¹）得出的内皮渗透系数如下：钠离子为2.9±0.8；乙二醇为7.3±1.5；1,3-丙二醇为7.9±3.2；1,2-丙二醇为10±4；1,4-丁二醇为14±8；1,5-戊二醇为21±6；1,6-己二醇为41±11；甲酰胺为16±9；乙酰胺为13±4；丙酰胺为31±12；丁酰胺为42±24；戊酰胺为79±12；氚化水为150±50。反向扩散校正范围从钠离子的8%到戊酰胺的75%。小的非电解质溶质的血液-组织被动交换的平行途径模型与这些结果相符，其中一个脂质途径通过内皮细胞，一个水性途径可能通过内皮间裂隙。
