Kume S, Muto A, Okano H, Mikoshiba K
Mikoshiba Calciosignal Net Project, Exploratory Research for Advanced Technology, Japan Science and Technology Corporation, Tokyo.
Mech Dev. 1997 Aug;66(1-2):157-68. doi: 10.1016/s0925-4773(97)00101-9.
To study the role of inositol 1,4,5-trisphosphate (IP3) receptors during early embryogenesis in Xenopus, we examined the temporal-spatial localization of Xenopus IP3 receptor (XIP3R). XIP3R protein is enriched in the animal hemisphere of early cleavage stage embryos and becomes localized in the ectoderm and involuted mesoderm in gastrula stage embryos. Up to tailbud stages, expression of XIP3R is observed in the mesodermal tissues and in most subregions of the central nervous system. A quantitative analysis of endogenous IP3 mass during normal early embryogenesis revealed an increase in IP3 mass first observed at early gastrula stage of 10.5 with an enrichment in the ectoderm throughout the gastrula stages, implying a potential role during gastrulation.
为了研究肌醇1,4,5 -三磷酸（IP3）受体在非洲爪蟾早期胚胎发育过程中的作用，我们检测了非洲爪蟾IP3受体（XIP3R）的时空定位。XIP3R蛋白在早期卵裂期胚胎的动物半球富集，并在原肠胚期胚胎中定位于外胚层和内卷中胚层。直至尾芽期，在中胚层组织和中枢神经系统的大多数亚区域都观察到了XIP3R的表达。对正常早期胚胎发育过程中内源性IP3含量的定量分析显示，IP3含量首先在原肠胚早期（10.5）增加，并在整个原肠胚阶段在外胚层富集，这意味着其在原肠胚形成过程中具有潜在作用。
