Sun E, Xu H, Liu Q, Zhou J, Zuo P, Wang J
Department of Chemistry, Huazhong University of Science and Technology, Wuhan, People's Republic of China.
Biol Trace Elem Res. 1995 Jun;48(3):239-50. doi: 10.1007/BF02789406.
Con A stimulated lymphocytes proliferation was measured as [3H]thymidine incorporation and IgG was quantified by single radial immunodiffusion to study recovering or protecting effect of selenium (Se) on immunity attacked by exogenous active oxygen species, H2O2 and 60Co-radiation, respectively. Lipid peroxidation was also determined to observe the relation between antioxidation ability and protecting ability of Se. It was found that H2O2 injured lymphocytes immunocompetence deeply and 60Co-radiation decreased immune response capacity greatly, but that administration of Se counteracts this damage. The antioxidative ability of Se was correlated with its protecting ability.
用[³H]胸腺嘧啶核苷掺入法测定刀豆蛋白A刺激的淋巴细胞增殖，并通过单向放射免疫扩散法定量IgG，以分别研究硒（Se）对外源性活性氧、H₂O₂和⁶⁰Co辐射攻击的免疫的恢复或保护作用。还测定了脂质过氧化，以观察硒的抗氧化能力与保护能力之间的关系。结果发现，H₂O₂严重损伤淋巴细胞免疫能力，⁶⁰Co辐射大大降低免疫反应能力，但给予硒可抵消这种损伤。硒的抗氧化能力与其保护能力相关。
