Olson G B, Anderson R E, Bartels P H
Hum Pathol. 1979 Mar;10(2):179-90. doi: 10.1016/s0046-8177(79)80007-6.
The radiosensitivities of lymphocytes of peripheral blood origin obtained from two healthy 45 year old male donors were studied simultaneously at yearly intervals over a three year period. Hypaque-Ficoll purified cells were exposed in vitro to 0, 5, 50, and 500 rads and then evaluated serially for viability of T and B cells, responsiveness to PHA and Con A, and morphologic evidence of injury as documented by standard light microscopy and computer assisted morphometric analysis. The results showed that T cells in both subjects were less radiosensitive than B cells. Differences between the two subjects also existed in the radiosensitivity of these two subpopulations of lymphocytes, differences that remained constant over the three years period of observation. The differences correlated with similar discrepancies in mitogenic responsiveness and are thought to relate to variations in the relative proportions of subpopulations of T and B cells. In the mouse, T and B cell subpopulations differ in radiosensitivity. The data reported herein are consistent with a similar situation in man.
在三年时间里，每年同时研究两名45岁健康男性供体的外周血淋巴细胞的放射敏感性。用聚蔗糖-泛影葡胺纯化的细胞在体外接受0、5、50和500拉德的照射，然后连续评估T细胞和B细胞的活力、对PHA和Con A的反应性，以及通过标准光学显微镜和计算机辅助形态计量分析记录的损伤形态学证据。结果显示，两名受试者的T细胞比B细胞对辐射更不敏感。这两个淋巴细胞亚群的放射敏感性在两名受试者之间也存在差异，这些差异在三年的观察期内保持不变。这些差异与促有丝分裂反应性的类似差异相关，并且被认为与T细胞和B细胞亚群的相对比例变化有关。在小鼠中，T细胞和B细胞亚群的放射敏感性不同。本文报道的数据与人类的类似情况一致。
