Jørgensen N, Auger J, Giwercman A, Irvine D S, Jensen T K, Jouannet P, Keiding N, Le Bon C, MacDonald E, Pekuri A M, Scheike T, Simonsen M, Suominen J, Skakkeboek N E
Department of Growth and Reproduction, Juliane Marie Center, National University Hospital, Copenhagen, Denmark.
Int J Androl. 1997 Aug;20(4):201-8. doi: 10.1046/j.1365-2605.1997.00052.x.
Some recent studies have indicated that sperm concentration has decreased during the last 50 years. However, comparisons between laboratories have revealed that geographical differences seem to exist and that any decrease may not be global. One point of criticism concerning comparison of results from different laboratories has been that some of the discrepancies detected could reflect the lack of standardized methods used in the different laboratories. Four teams, each consisting of one physician and one technician from groups which have recently published data on semen quality, met in order to evaluate the variability between their laboratories on semen analysis. Twenty-six fresh semen samples from unselected men were analysed. The groups analysed the samples according to the normal practice in their laboratories, using their own equipment. The variation between laboratories was estimated through a random effects model. For sperm concentration and semen volume assessment a remarkable consistency between laboratories was detected, in contrast to the very considerable inter-individual variation. For sperm motility and morphology assessments interlaboratory consistency was much poorer. In conclusion, evaluation of sperm motility and morphology characteristics requires further standardization in order to achieve comparable data from different laboratories. However, semen volume and sperm concentration are characteristics which can be compared reliably between laboratories, when similar methodologies are used.
最近的一些研究表明，在过去50年里精子浓度有所下降。然而，实验室之间的比较显示，似乎存在地域差异，而且任何下降可能并非全球性的。关于不同实验室结果比较的一个批评点在于，检测到的一些差异可能反映出不同实验室使用的方法缺乏标准化。来自最近发表过精液质量数据的小组的四个团队，每个团队由一名医生和一名技术人员组成，他们会面以评估各自实验室在精液分析方面的差异。对26份来自未经过挑选男性的新鲜精液样本进行了分析。这些小组根据各自实验室的常规做法，使用自己的设备对样本进行分析。通过随机效应模型估计实验室之间的差异。对于精子浓度和精液体积评估，检测到实验室之间有显著的一致性，这与个体间非常大的差异形成对比。对于精子活力和形态评估，实验室间的一致性要差得多。总之，为了从不同实验室获得可比数据，精子活力和形态特征的评估需要进一步标准化。然而，当使用相似方法时，精液体积和精子浓度是可以在实验室之间可靠比较的特征。
