Dunphy B C, Kay R, Barratt C L, Cooke I D
Harris Birthright Centre for Reproductive Medicine, Jessop Hospital for Women, Sheffield, United Kingdom.
J Androl. 1989 Sep-Oct;10(5):378-85. doi: 10.1002/j.1939-4640.1989.tb00124.x.
Intra- and inter-technician variability in assessing sperm motility by the methods recently advocated by the World Health Organization (WHO) were studied. The intra- and inter-technician variability in estimating sperm concentration and the intra-technician variability in assessing sperm morphology were also examined. Intra-technician variability in assessing sperm motility appeared to be related to the natural ability and/or training of the observer. Although in general the intra-technician variability was low, there were marked and clinically significant differences between observers when assessing the same semen sample. There was no significant difference between observers in the assessment of sperm concentration, and intra-technician variability was low. When assessing sperm morphology, the intra-technician variability was potentially large (above a level of 20% morphologically ideal spermatozoa). Technicians should be recruited who have natural ability as observers. Quality control appears to be an essential exercise for any center that plans to relate semen parameters to fertility outcome.
研究了技术人员内部和技术人员之间采用世界卫生组织（WHO）最近倡导的方法评估精子活力时的变异性。还检查了技术人员内部和技术人员之间估计精子浓度的变异性以及技术人员内部评估精子形态的变异性。技术人员内部评估精子活力的变异性似乎与观察者的天赋能力和/或培训有关。虽然总体而言技术人员内部变异性较低，但在评估相同精液样本时，观察者之间存在显著且具有临床意义的差异。在评估精子浓度方面，观察者之间没有显著差异，技术人员内部变异性较低。在评估精子形态时，技术人员内部变异性可能很大（形态学上理想的精子超过20%的水平）。应招募具有观察天赋能力的技术人员。对于任何计划将精液参数与生育结果相关联的中心来说，质量控制似乎是一项必不可少的工作。
