八个睾丸特异性基因在小鼠染色体上的定位。
Mapping of eight testis-specific genes to mouse chromosomes.
作者信息
Taketo M M, Araki Y, Matsunaga A, Yokoi A, Tsuchida J, Nishina Y, Nozaki M, Tanaka H, Koga M, Uchida K, Matsumiya K, Okuyama A, Rochelle J M, Nishimune Y, Matsui M, Seldin M F
机构信息
Laboratory of Biomedical Genetics, Graduate School of Pharmaceutical Sciences, University of Tokyo, Japan.
出版信息
Genomics. 1997 Nov 15;46(1):138-42. doi: 10.1006/geno.1997.5014.
We previously identified eight testis-specific genes using antibodies raised against testicular germ cells. They are expressed during spermatogenesis and are presumed to be involved in testicular germ cell differentiation and sperm formation. We have mapped the genomic loci for these testis-specific genes using restriction fragment length variants in interspecific backcross mice. The calmegin gene (Clgn) was mapped to Chr 8. The synaptonemal complex protein gene 1 (Sycp1) probe hybridized with two sequences on different chromosomes; Sycp1-rs2 was mapped to Chr 3, whereas Sycp1-rs3 was mapped to Chr 7. The relaxin-like factor gene (Rlnl) was mapped to Chr 8, and collapsin response mediator protein 1 (Crmp1) was mapped to Chr 5. Three novel genes encoding testis-specific proteins A2 (Tsga2), A8 (Tsga8), and A12 (Tsga12) were mapped to chromosomes 3, X, and 10, respectively.
我们之前利用针对睾丸生殖细胞产生的抗体鉴定出了八个睾丸特异性基因。它们在精子发生过程中表达，并且据推测参与睾丸生殖细胞分化和精子形成。我们利用种间回交小鼠中的限制性片段长度变异，绘制了这些睾丸特异性基因的基因组位点。钙牵蛋白基因（Clgn）定位于8号染色体。联会复合体蛋白基因1（Sycp1）探针与不同染色体上的两个序列杂交；Sycp1-rs2定位于3号染色体，而Sycp1-rs3定位于7号染色体。松弛素样因子基因（Rlnl）定位于8号染色体，塌陷反应介导蛋白1（Crmp1）定位于5号染色体。三个编码睾丸特异性蛋白A2（Tsga2）、A8（Tsga8）和A12（Tsga12）的新基因分别定位于3号、X号和10号染色体。
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