Doetsch F, Alvarez-Buylla A
Rockefeller University, New York, NY 10021, USA.
Proc Natl Acad Sci U S A. 1996 Dec 10;93(25):14895-900. doi: 10.1073/pnas.93.25.14895.
Cells in the brains of adult mammals continue to proliferate in the subventricular zone (SVZ) throughout the lateral wall of the lateral ventricle. Here we show, using whole mount dissections of this wall from adult mice, that the SVZ is organized as an extensive network of chains of neuronal precursors. These chains are immunopositive to the polysialylated form of NCAM, a molecule present at sites of plasticity, and TuJ1, an early neuronal marker. The majority of the chains are oriented along the rostrocaudal axis and many join the rostral migratory stream that terminates in the olfactory bulb. Using focal microinjections of DII and transplantation of SVZ cells carrying a neuron-specific reporter gene, we demonstrate that cells originating at different rostrocaudal levels of the SVZ migrate rostrally and reach the olfactory bulb where they differentiate into neurons. Our results reveal an extensive network of pathways for the tangential chain migration of neuronal precursors throughout the lateral wall of the lateral ventricle in the adult mammalian brain.
成年哺乳动物大脑中的细胞在侧脑室整个侧壁的脑室下区（SVZ）持续增殖。在此我们利用成年小鼠该脑壁的整装解剖显示，SVZ组织成一个由神经元前体细胞链构成的广泛网络。这些细胞链对多唾液酸形式的神经细胞黏附分子（NCAM，一种存在于可塑性部位的分子）以及早期神经元标志物TuJ1呈免疫阳性。大多数细胞链沿头尾轴排列，许多细胞链汇入终止于嗅球的头向迁移流。通过局部微量注射DII以及移植携带神经元特异性报告基因的SVZ细胞，我们证明起源于SVZ不同头尾水平的细胞向头侧迁移并到达嗅球，在那里它们分化为神经元。我们的结果揭示了成年哺乳动物大脑中神经元前体细胞在侧脑室整个侧壁进行切向链迁移的广泛通路网络。
