Desmarchelier C, Witting Schaus F, Coussio J, Cicca G
Cátedra de Microbiología Industrial y Biotecnología: Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Argentina.
J Ethnopharmacol. 1997 Oct;58(2):103-8. doi: 10.1016/s0378-8741(97)00087-1.
The total reactive antioxidant potential (TRAP) of 'Sangre de Drago' from Croton lechleri (Euphorbiaceae) was determined by monitoring the intensity of luminol enhanced chemiluminescence enhanced by peroxyl radicals derived from thermolysis of 2,2'-azo-bis(2-amidinopropane). The TRAP index was calculated as 935.4 +/- 141 microM, measured as equivalents of Trolox concentration. On the other hand, the additive incorporation of lower concentrations yielded an instantaneous increase in chemiluminescence, suggesting a prooxidant activity at these levels. DNA sugar damage induced by Fe(II) salts was also used to determine the capacity of the latex to suppress hydroxyl radical-mediated degradation of DNA. As in the case of luminol enhanced chemiluminescence, Sangre de Drago was highly effective in reducing oxidation of DNA at higher concentrations, but showed an increase in the production of TBARS at lower doses, as compared to the control. Finally, antioxidant activity was tested using hydroperoxide-initiated chemiluminescence in rat liver homogenates, and the latex showed an increase in light emission, suggesting the presence of prooxidant compounds.
通过监测由2,2'-偶氮双(2-脒基丙烷)热解产生的过氧自由基增强的鲁米诺增强化学发光强度，测定了大戟科巴豆属植物‘龙血’的总反应性抗氧化潜力(TRAP)。TRAP指数计算为935.4±141微摩尔，以Trolox浓度当量表示。另一方面，较低浓度的添加剂加入导致化学发光瞬间增加，表明在这些水平上具有促氧化活性。亚铁盐诱导的DNA糖损伤也用于测定乳胶抑制羟基自由基介导的DNA降解的能力。与鲁米诺增强化学发光情况一样，‘龙血’在较高浓度下能非常有效地减少DNA氧化，但与对照相比在较低剂量下丙二醛产量增加。最后，在大鼠肝脏匀浆中用过氧化氢引发的化学发光测试抗氧化活性，乳胶显示发光增加，表明存在促氧化化合物。
