Titlestad K, Georgsen J, Andersen H, Kristensen T
Department of Clinical Immunology, Odense University Hospital, Denmark.
Vox Sang. 1997;73(4):246-51. doi: 10.1046/j.1423-0410.1997.7340246.x.
The purpose of this study was to evaluate more than 3 years of experience with a gel technique in combination with pooled screening cells for the detection of irregular red cell antibodies.
Conventional serologic methods were used for blood typing, antibody screening and cross-matching until the end of 1992. We introduced the gel technique as a routine assay for antibody detection and identification in 1993.
After the tube technique had been abandoned, the number of false-positive antibody screening tests was reduced by 71%, positive antibody screening tests by 33%, enzyme agglutination by 100% and rouleaux reactions and cold-reacting antibodies by more than 50%. There was a 40% increase in first-time detection of clinically relevant antibodies. We saw no increase in delayed haemolytic transfusion reactions.
For the detection of irregular red cell antibodies, pooled screening cells in combination with a gel technique are at least as efficient and safe as a conventional tube technique with unpooled test cells.
本研究旨在评估凝胶技术结合混合筛查细胞检测不规则红细胞抗体超过3年的经验。
在1992年底前，采用传统血清学方法进行血型鉴定、抗体筛查和交叉配血。1993年，我们引入凝胶技术作为抗体检测和鉴定的常规检测方法。
摒弃试管技术后，抗体筛查假阳性试验次数减少了71%，阳性抗体筛查试验减少了33%，酶凝集减少了100%，缗钱状反应和冷反应抗体减少了50%以上。临床相关抗体的首次检测增加了40%。我们未发现迟发性溶血性输血反应增加。
对于不规则红细胞抗体的检测，混合筛查细胞结合凝胶技术至少与使用未混合检测细胞的传统试管技术一样高效和安全。
