Nelson B, Calvo W, Fliedner T M, Herbst E, Bruch C, Schnappauf H P, Flad H D
Am J Pathol. 1976 Aug;84(2):259-82.
Fresh and cryopreserved autologous or allogeneic mononuclear blood cells (MBCs) intravenously injected in 1200 R total-body x-irradiated dogs repopulated lymph nodes within 10 days after tranfusion. Several parameters of the lymphopoietic regeneration were correlated with the number of cells transfused and with the number of colony-forming units contained in the cell suspension when they were cultured in agar (CFUc). Values within the normal or close to normal range were reached in the mesenteric nodes of most of the animals transfused with 10 X 10(9) MBC or more. These values were obtained when 5 X 10(5) CFUc or more were transfused. Axillary nodes showed lower values than mesenteric nodes. They were mostly under the normal range but well over those of the irradiated controls. Frozen and thawed MBCs seem to be as effective as fresh cells for lymphopoietic restoration. The mesenteric nodes of dogs transfused with allogeneic MBCs showed higher cellularity and larger cortical-paracortical areas than those of dogs tranfused with approximately the same number of autologous cells. The repopulation of lymph nodes parallels that of the marrow.
给接受1200拉德全身X射线照射的犬静脉注射新鲜的和冷冻保存的自体或异体单核血细胞（MBCs）后，这些细胞在输血后10天内使淋巴结重新细胞化。当在琼脂中培养时，淋巴细胞再生的几个参数与输入的细胞数量以及细胞悬液中所含集落形成单位（CFUc）的数量相关。大多数输入10×10⁹个或更多MBCs的动物，其肠系膜淋巴结的值达到正常或接近正常范围。当输入5×10⁵个或更多CFUc时可获得这些值。腋窝淋巴结的值低于肠系膜淋巴结。它们大多低于正常范围，但远高于照射对照组。冷冻和解冻的MBCs在淋巴细胞恢复方面似乎与新鲜细胞一样有效。输入异体MBCs的犬的肠系膜淋巴结比输入大致相同数量自体细胞的犬的肠系膜淋巴结具有更高的细胞密度和更大的皮质-副皮质区域。淋巴结的重新细胞化与骨髓的情况相似。
