嗜热脂肪芽孢杆菌醛缩酶中的金属置换研究以及I类和II类醛缩酶作用机制的比较。
Metal-replacement studies in Bacillus stearothermophilus aldolase and a comparison of the mechanisms of class I and class II aldolases.
作者信息
Hill H A, Lobb R R, Sharp S L, Stokes A M, Harris J I, Jack R S
出版信息
Biochem J. 1976 Mar 1;153(3):551-60. doi: 10.1042/bj1530551.
Abstract
A comparison of the product-inhibition patterns during cleavage of D-fructose 1,6-diphosphate by aldolases from yeast, rabbit muscle and Bacillus stearothermophilus shows an ordered reaction sequence for all three enzymes, with dihydroxyacetone phosphate the last-leaving product. Addition of Zn2+, Co2+, Fe2+, Mn2+ or Cd2+ ions to the inactive apo-(Bacillus stearothermophilus aldolase) restores activity to different extents, whereas Ni2+, Mg2+ or Cu2+ ions have no effect. The cleavage activity of this aldolase is not enhanced by added K+ ion. The effects of metal replacement on thermal stability, Km and Vmax. are given and the possible role of the metal is discussed in the light of these results.
摘要
对酵母、兔肌肉和嗜热脂肪芽孢杆菌的醛缩酶在裂解1,6-二磷酸-D-果糖过程中的产物抑制模式进行比较，结果表明这三种酶的反应顺序均为有序反应，磷酸二羟丙酮是最后离开的产物。向无活性的脱辅基（嗜热脂肪芽孢杆菌醛缩酶）中添加Zn2+、Co2+、Fe2+、Mn2+或Cd2+离子可不同程度地恢复其活性，而Ni2+、Mg2+或Cu2+离子则无此作用。添加K+离子不会增强该醛缩酶的裂解活性。给出了金属置换对热稳定性、Km和Vmax的影响，并根据这些结果讨论了金属可能发挥的作用。
相似文献
1
Metal-replacement studies in Bacillus stearothermophilus aldolase and a comparison of the mechanisms of class I and class II aldolases.嗜热脂肪芽孢杆菌醛缩酶中的金属置换研究以及I类和II类醛缩酶作用机制的比较。
Biochem J. 1976 Mar 1;153(3):551-60. doi: 10.1042/bj1530551.
2
Inhibition of fructose-1,6-bisphosphate aldolase from rabbit muscle and Bacillus stearothermophilus.兔肌肉和嗜热脂肪芽孢杆菌中果糖-1,6-二磷酸醛缩酶的抑制作用
Eur J Biochem. 1977 Oct 17;80(1):119-33. doi: 10.1111/j.1432-1033.1977.tb11864.x.
3
The proton exchange of the pro-S hydrogen atom at C-1 in dihydroxyacetone phosphate and D-fructose 1,6-bisphosphate catalysed by class-I and class-II aldolases.I类和II类醛缩酶催化磷酸二羟丙酮和D-果糖1,6-二磷酸中C-1位前手性氢原子的质子交换。
Biochem J. 1978 Jun 1;171(3):539-42. doi: 10.1042/bj1710539.
4
Evaluation of four microbial Class II fructose 1,6-bisphosphate aldolase enzymes for use as biocatalysts.四种微生物II类果糖1,6-二磷酸醛缩酶作为生物催化剂的评估。
Protein Expr Purif. 2011 Dec;80(2):224-33. doi: 10.1016/j.pep.2011.06.020. Epub 2011 Jul 7.
5
The crystal structure of a class II fructose-1,6-bisphosphate aldolase shows a novel binuclear metal-binding active site embedded in a familiar fold.II类果糖-1,6-二磷酸醛缩酶的晶体结构显示出一个嵌入常见折叠结构中的新型双核金属结合活性位点。
Structure. 1996 Nov 15;4(11):1303-15. doi: 10.1016/s0969-2126(96)00138-4.
6
The effect of divalent cations on bovine spermatozoal adenylate cyclase activity.二价阳离子对牛精子腺苷酸环化酶活性的影响。
J Cyclic Nucleotide Res. 1975;1(6):271-81.
7
Enzymatic properties of fish muscle aldolase.鱼肌肉醛缩酶的酶学性质
Comp Biochem Physiol B. 1989;92(2):405-10. doi: 10.1016/0305-0491(89)90301-5.
8
Classification of fructose-1,6-bisphosphate aldolases based on 18O retention in the cleavage reaction.基于1,6-二磷酸果糖醛缩酶在裂解反应中对18O的保留情况进行的分类
Biochim Biophys Acta. 1975 Oct 22;403(2):563-72. doi: 10.1016/0005-2744(75)90085-6.
9
Kinetic properties of fructose bisphosphate aldolase from Trypanosoma brucei compared to aldolase from rabbit muscle and Staphylococcus aureus.布氏锥虫果糖二磷酸醛缩酶与兔肌肉和金黄色葡萄球菌醛缩酶的动力学特性比较
Mol Biochem Parasitol. 1991 Jul;47(1):1-9. doi: 10.1016/0166-6851(91)90142-s.
10
Raman spectroscopy of DNA-metal complexes. II. The thermal denaturation of DNA in the presence of Sr2+, Ba2+, Mg2+, Ca2+, Mn2+, Co2+, Ni2+, and Cd2+.DNA-金属络合物的拉曼光谱。II. Sr2+、Ba2+、Mg2+、Ca2+、Mn2+、Co2+、Ni2+和Cd2+存在下DNA的热变性
Biophys J. 1995 Dec;69(6):2623-41. doi: 10.1016/S0006-3495(95)80133-5.
引用本文的文献
1
Potentiating Activity of GmhA Inhibitors on Gram-Negative Bacteria.GmhA抑制剂对革兰氏阴性菌的增效活性。
J Med Chem. 2024 Apr 25;67(8):6610-6623. doi: 10.1021/acs.jmedchem.4c00037. Epub 2024 Apr 10.
2
Identification of arginine 331 as an important active site residue in the class II fructose-1,6-bisphosphate aldolase of Escherichia coli.鉴定精氨酸331是大肠杆菌II类果糖-1,6-二磷酸醛缩酶中的一个重要活性位点残基。
Protein Sci. 1996 Jan;5(1):154-61. doi: 10.1002/pro.5560050119.
3
Purification and characterization of the class-II D-fructose 1,6-bisphosphate aldolase from Escherichia coli (Crookes' strain).来自大肠杆菌（克鲁克斯菌株）的II类D-果糖1,6-二磷酸醛缩酶的纯化与特性分析
Biochem J. 1978 Mar 1;169(3):633-41. doi: 10.1042/bj1690633.
4
The proton exchange of the pro-S hydrogen atom at C-1 in dihydroxyacetone phosphate and D-fructose 1,6-bisphosphate catalysed by class-I and class-II aldolases.I类和II类醛缩酶催化磷酸二羟丙酮和D-果糖1,6-二磷酸中C-1位前手性氢原子的质子交换。
Biochem J. 1978 Jun 1;171(3):539-42. doi: 10.1042/bj1710539.
5
13C nuclear magnetic resonance studies of anaerobic glycolysis in suspensions of yeast cells.酵母细胞悬液中无氧糖酵解的13C核磁共振研究。
Proc Natl Acad Sci U S A. 1979 Dec;76(12):6096-100. doi: 10.1073/pnas.76.12.6096.
本文引用的文献
1
Comparative properties of yeast and muscle aldolase.酵母醛缩酶与肌肉醛缩酶的比较特性
J Biol Chem. 1961 Dec;236:3185-92.
2
MECHANISM OF THE ALDOLASE REACTION.醛缩酶反应的机制
J Biol Chem. 1965 Apr;240:1758-65.
3
DISTINCTIVE PROPERTIES OF NATIVE AND CARBOXYPEPTIDASE-TREATED ALDOLASES OF RABBIT MUSCLE AND LIVER.兔肌肉和肝脏中天然型及羧肽酶处理型醛缩酶的独特性质。
J Biol Chem. 1965 Mar;240:1327-37.
4
EVOLUTION OF ALDOLASE.醛缩酶的进化
Fed Proc. 1964 Nov-Dec;23:1248-57.
5
THE MECHANISM OF ACTION OF ALDOLASES. IV. LYSINE AS THE SUBSTRATE-BINDING SITE.醛缩酶的作用机制。IV. 赖氨酸作为底物结合位点。
Biochem Z. 1963;338:36-51.
6
Interaction between rabbit muscle aldolase and dihydroxyacetone phosphate.兔肌肉醛缩酶与磷酸二羟丙酮之间的相互作用。
J Biol Chem. 1963 Jan;238:105-7.
7
Studies on the mechanism on the aldolase reaction; isotope exchange reactions of muscle and yeast aldolase.醛缩酶反应机制的研究；肌肉和酵母醛缩酶的同位素交换反应。
J Biol Chem. 1958 Mar;231(1):315-29.
8
Contamination in trace element analysis and its control.微量元素分析中的污染及其控制。
Methods Biochem Anal. 1957;5:273-335. doi: 10.1002/9780470110218.ch6.
9
Fructose diphosphate aldolase from Fusarium oxysporum f. lycopersici. 3. Studies on the mechanism of reaction.尖孢镰刀菌番茄专化型的果糖二磷酸醛缩酶。3. 反应机制研究。
Can J Biochem. 1967 Dec;45(12):1909-17. doi: 10.1139/o67-224.
10
Aldolase reaction with sugar diphosphates.醛缩酶与二磷酸糖的反应。
Science. 1967 Mar 3;155(3766):1101-3. doi: 10.1126/science.155.3766.1101.
Zotero 插件