Fallon A, Bradley J F, Burns J, O'D McGee J
J Clin Pathol. 1984 May;37(5):542-8. doi: 10.1136/jcp.37.5.542.
Four factors which stimulate collagen synthesis and prolyl hydroxylase activity in cultures of human and mouse fibroblasts have been isolated by molecular sieve chromatography from animal and human fibrotic and cirrhotic livers. These factors do not stimulate protein or DNA synthesis or total DNA in these cultures. It has also been shown that these factors, designated collagen stimulating factors F1-F4, do not owe their activity to ascorbate or glutamine. Collagen stimulating factors are heat stable, and F1 and F2 have apparent molecular weights of about 4000 and 1000 respectively. Since these factors are not present in normal animal or human liver it is suggested that they may be responsible for increased collagen production in vivo in hepatic fibrosis and cirrhosis.
通过分子筛色谱法，已从动物及人类的纤维化和肝硬化肝脏中分离出四种可刺激人及小鼠成纤维细胞培养物中胶原蛋白合成和脯氨酰羟化酶活性的因子。这些因子不会刺激这些培养物中的蛋白质或DNA合成，也不会刺激总DNA合成。研究还表明，这些被命名为胶原蛋白刺激因子F1 - F4的因子，其活性并非源于抗坏血酸或谷氨酰胺。胶原蛋白刺激因子具有热稳定性，F1和F2的表观分子量分别约为4000和1000。由于这些因子在正常动物或人类肝脏中不存在，因此有人提出，它们可能是导致肝纤维化和肝硬化体内胶原蛋白产生增加的原因。
