Kong Saerom, Choi Hye-Ryung, Kim Yoon-Jeong, Lee Yoon-Sik, Park Kyoung-Chan, Kwak Seon-Yeong
School of Chemical and Biological Engineering, Seoul National University, Seoul 08826, Korea.
Department of Dermatology, Seoul National University Bundang Hospital, Seongnam 13620, Korea.
Antioxidants (Basel). 2020 Nov 10;9(11):1106. doi: 10.3390/antiox9111106.
Excessive accumulation of melanin can cause skin pigmentation disorders, which may be accompanied by significant psychological stress. Although many natural and synthetic products have been developed for the regulation of melanogenesis biochemistry, the management of unwanted skin pigmentation remains challenging. Herein, we investigated the potential hypopigmenting properties of peptide sequences that originated from milk proteins such as ĸ-casein and β-lactoglobulin. These proteins are known to inhibit melanogenesis and their hydrolysates are reported as antioxidant peptides. We synthesize tetrapeptide fragments of the milk protein hydrolysates and investigate the amino acids that are essential for designing peptides with tyrosinase inhibitory and antioxidant activities. We found that the peptide methionine-histidine-isoleucine-arginine amide sufficiently inhibits mushroom tyrosinase activity, shows potent antioxidant activity and effectively impedes melanogenesis in cultured melanocytes via cooperative biological activities. Our findings demonstrate the potential utility of the bioactive tetrapeptide from milk proteins as a chemical alternative to hypopigmenting agents.
黑色素的过度积累会导致皮肤色素沉着紊乱，这可能会伴随着巨大的心理压力。尽管已经开发出许多天然和合成产品用于调节黑色素生成的生物化学过程，但对 unwanted 皮肤色素沉着的管理仍然具有挑战性。在此，我们研究了源自牛奶蛋白（如 κ-酪蛋白和 β-乳球蛋白）的肽序列的潜在美白特性。已知这些蛋白质可抑制黑色素生成，并且它们的水解产物被报道为抗氧化肽。我们合成了牛奶蛋白水解产物的四肽片段，并研究了设计具有酪氨酸酶抑制和抗氧化活性的肽所必需的氨基酸。我们发现肽甲硫氨酸-组氨酸-异亮氨酸-精氨酸酰胺充分抑制蘑菇酪氨酸酶活性，显示出强大的抗氧化活性，并通过协同生物活性有效地阻碍培养的黑素细胞中的黑色素生成。我们的研究结果证明了来自牛奶蛋白的生物活性四肽作为美白剂的化学替代品的潜在用途。
