Suzuki K, Aoki K, Ohya K
Department of Oral and Maxillofacial Surgery, Graduate School of Dentistry, Tokyo Medical and Dental University, Japan.
Bone. 1997 Dec;21(6):507-14. doi: 10.1016/s8756-3282(97)00204-4.
We investigated the bone remodeling activity on titanium implants with different surface roughnesses using a confocal laser scanning microscope (CLSM). Two kinds of implants were used, the machined smooth-surfaced titanium and the plasma-sprayed rough-surfaced titanium. These implants were randomly inserted in a rabbit's femur from the lateral aspect of the diaphysis bicortically. Rabbits were killed at 6, 16, and 42 weeks after surgery. The implant-bone blocks were embedded in polyester resin, and were prepared to make undecalcified ground sections. Histomorphometric analyses were performed at the cortical bone-implant interface using the image obtained by CLSM. Percentages of direct bone-implant contact and bone volume (BV/TV) around the implant was greater in rough-surfaced titanium compared with the smooth-surfaced titanium at 42 weeks after implantation. On the contrary, the eroded surface (ES/BS) appeared to be less in the rough-surfaced titanium than in the smooth-surfaced titanium at 6 weeks after implantation, but thereafter, no difference was found between the two kinds of implants. Mineralizing surface (MS/BS) and mineral apposition rate (MAR) showed no significant differences throughout the experimental period. These results indicate that increased bone volume in the rabbits of rough-surfaced titanium implants is due to less remodeling activity during the early stage after implantation compared with the smooth-surfaced implants. The surface roughness of titanium is one factor which helps in determining the balance between bone formation and resorption of remodeling at the interface of the bone implants.
我们使用共聚焦激光扫描显微镜（CLSM）研究了不同表面粗糙度的钛植入物上的骨重塑活性。使用了两种植入物，即机械加工的光滑表面钛植入物和等离子喷涂的粗糙表面钛植入物。这些植入物从骨干的外侧双皮质地随机插入兔股骨中。在手术后6、16和42周处死兔子。将植入物-骨块嵌入聚酯树脂中，并制备未脱钙的磨片。使用CLSM获得的图像在皮质骨-植入物界面进行组织形态计量学分析。与光滑表面钛相比，植入后42周时，粗糙表面钛植入物周围的直接骨-植入物接触百分比和骨体积（BV/TV）更高。相反，在植入后6周时，粗糙表面钛的侵蚀表面（ES/BS）似乎比光滑表面钛少，但此后，两种植入物之间未发现差异。在整个实验期间，矿化表面（MS/BS）和矿化沉积率（MAR）均无显著差异。这些结果表明，与光滑表面植入物相比，粗糙表面钛植入物兔子的骨体积增加是由于植入后早期的重塑活性较低。钛的表面粗糙度是有助于确定骨植入物界面处骨形成和重塑吸收之间平衡的一个因素。
