Noguchi N, Yamashita H, Gotoh N, Yamamoto Y, Numano R, Niki E
Research Center for Advanced Science and Technology, University of Tokyo, Meguro, Japan.
Free Radic Biol Med. 1998 Jan 15;24(2):259-68. doi: 10.1016/s0891-5849(97)00230-x.
Both hydrophilic and hydrophobic azo radical initiators are useful for in vitro studies on lipid peroxidation and its inhibition by antioxidants. In the present study, a new lipophilic azo compound, 2,2'-azobis(4-methoxy-2,4-dimethylvaleronitrile) (MeO-AMVN), was introduced and its action as an initiator of lipid peroxidation was examined. MeO-AMVN decomposed about 15 times as fast as 2,2'-azobis(2,4-dimethylvaleronitrile) (AMVN), a widely used lipophilic azo initiator, and MeO-AMVN-initiated free radical-mediated peroxidations of lipids in organic solution and in micelles, membranes, and low-density lipoprotein in aqueous dispersions with much smaller concentration than AMVN. The rate of chain initiation by MeO-AMVN varied significantly with the medium and decreased with increasing viscosity of the medium. The advantage and cautions for using MeO-AMVN as a lipophilic radical source have been discussed and it has been concluded that MeO-AMVN, when properly used, is a useful radical initiator of lipid peroxidations especially in micelles, membranes, and lipoproteins.
亲水性和疏水性偶氮自由基引发剂都可用于脂质过氧化及其抗氧化剂抑制作用的体外研究。在本研究中，引入了一种新的亲脂性偶氮化合物2,2'-偶氮双(4-甲氧基-2,4-二甲基戊腈)(MeO-AMVN)，并研究了其作为脂质过氧化引发剂的作用。MeO-AMVN的分解速度约为广泛使用的亲脂性偶氮引发剂2,2'-偶氮双(2,4-二甲基戊腈)(AMVN)的15倍，并且MeO-AMVN在有机溶液、胶束、膜以及水相分散体中的低密度脂蛋白中引发脂质的自由基介导过氧化反应时所需浓度比AMVN小得多。MeO-AMVN引发链的速率随介质的不同而有显著变化，并随介质粘度的增加而降低。本文讨论了使用MeO-AMVN作为亲脂性自由基源的优点和注意事项，并得出结论：如果使用得当，MeO-AMVN是脂质过氧化反应中一种有用的自由基引发剂，尤其是在胶束、膜和脂蛋白中。
