Lu P, Davis B P, Jeffries T W
Forest Products Laboratory, USDA Forest Service, Madison, Wisconsin 53705-2398, USA.
Appl Environ Microbiol. 1998 Jan;64(1):94-7. doi: 10.1128/AEM.64.1.94-97.1998.
In Pichia stipitis, fermentative and pyruvate decarboxylase (PDC) activities increase with diminished oxygen rather than in response to fermentable sugars. To better characterize PDC expression and regulation, two genes for PDC (PsPDC1 and PsPDC2) were cloned and sequenced from P. stipitis CBS 6054. Aside from Saccharomyces cerevisiae, from which three PDC genes have been characterized, P. stipitis is the only organism from which multiple genes for PDC have been identified and characterized. PsPDC1 and PsPDC2 have diverged almost as far from one another as they have from the next most closely related known yeast gene. PsPDC1 contains an open reading frame of 1,791 nucleotides encoding 597 amino acids. PsPDC2 contains a reading frame of 1,710 nucleotides encoding 570 amino acids. An 81-nucleotide segment in the middle of the beta domain of PsPDC1 codes for a unique segment of 27 amino acids, which may play a role in allosteric regulation. The 5' regions of both P. stipitis genes include two putative TATA elements that make them similar to the PDC genes from S. cerevisiae, Kluyveromyces marxianus, and Hanseniaspora uvarum.
在树干毕赤酵母中，发酵活性和丙酮酸脱羧酶（PDC）活性会随着氧气减少而增加，而非对可发酵糖做出反应。为了更好地表征PDC的表达和调控，从树干毕赤酵母CBS 6054中克隆并测序了两个PDC基因（PsPDC1和PsPDC2）。除了已鉴定出三个PDC基因的酿酒酵母外，树干毕赤酵母是唯一已鉴定和表征多个PDC基因的生物体。PsPDC1和PsPDC2之间的差异程度几乎与它们与下一个最密切相关的已知酵母基因之间的差异程度相同。PsPDC1包含一个1791个核苷酸的开放阅读框，编码597个氨基酸。PsPDC2包含一个1710个核苷酸的阅读框，编码570个氨基酸。PsPDC1的β结构域中间的一个81个核苷酸的片段编码一个27个氨基酸的独特片段，该片段可能在变构调节中发挥作用。树干毕赤酵母这两个基因的5'区域都包含两个推定的TATA元件，这使得它们与酿酒酵母、马克斯克鲁维酵母和葡萄汁有孢汉逊酵母的PDC基因相似。
