Passoth V, Zimmermann M, Klinner U
Institut für Biologie IV (Mikrobiologie), RWTH Aachen, Germany.
Appl Biochem Biotechnol. 1996 Spring;57-58:201-12. doi: 10.1007/BF02941701.
The respiration of Pichia stipitis was not repressed by either high concentrations of fermentable sugars or oxygen limitation. Fermentation was not induced by high sugar concentrations, but was inactivated by aerobic conditions. The activity of pyruvate dehydrogenase was constitutive. In contrast, pyruvate decarboxylase, alcohol dehydrogenase, and aldehyde dehydrogenase were induced by a reduction in the oxygen tension. It was demonstrated that in P. stipitis, the pyruvate decarboxylase is not induced by a signal from glycolysis. Contrary to Saccharomyces cerevisiae, the pyruvate decarboxylase was not inhibited by phosphate.
树干毕赤酵母的呼吸作用不会受到高浓度可发酵糖或氧气限制的抑制。高糖浓度不会诱导发酵，但有氧条件会使其失活。丙酮酸脱氢酶的活性是组成型的。相比之下，丙酮酸脱羧酶、乙醇脱氢酶和乙醛脱氢酶是由氧张力降低诱导产生的。结果表明，在树干毕赤酵母中，丙酮酸脱羧酶不是由糖酵解信号诱导产生的。与酿酒酵母相反，丙酮酸脱羧酶不受磷酸盐的抑制。
