Improvement of the immunomagnetic separation method selective for Escherichia coli O157 strains.
作者信息
Tomoyasu T
机构信息
Department of Microbiology, Kobe Institute of Health, Japan.
出版信息
Appl Environ Microbiol. 1998 Jan;64(1):376-82. doi: 10.1128/AEM.64.1.376-382.1998.
Abstract
Immunomagnetic separation is a useful enrichment method selective for Escherichia coli O157 cells against non-O157 E. coli cells from a preenrichment culture. However, E. coli cells are adsorbed onto a solid surface nonspecifically. With the conventional immunomagnetic separation method, this nonspecific adsorption interfered with immunomagnetic separation. It was found that this interference could be reduced with a low-ionic-strength solution. When immunomagnetic separation was carried out with this solution, the proportion of E. coli O157 cells to non-O157 E. coli cells increased from 9.6 to 31.4 times compared to the proportion obtained by the conventional immunomagnetic separation method. The effectiveness of this solution was successfully evaluated by the use of E. coli O157-spiked samples.
摘要
相似文献
1
Improvement of the immunomagnetic separation method selective for Escherichia coli O157 strains.
Appl Environ Microbiol. 1998 Jan;64(1):376-82. doi: 10.1128/AEM.64.1.376-382.1998.
2
Comparison of rapid enzyme-linked immunosorbent assay and immunomagnetic separation methods for detection of Escherichia coli O157 in fecal, hide, carcass, and ground beef samples.
J Food Prot. 2007 Oct;70(10):2230-4. doi: 10.4315/0362-028x-70.10.2230.
3
Detection and isolation of low levels of E. coli O157:H7 in cilantro by real-time PCR, immunomagnetic separation, and cultural methods with and without an acid treatment.
J Food Sci. 2012 Aug;77(8):M481-9. doi: 10.1111/j.1750-3841.2012.02813.x.
4
Development of an immunomagnetic bead-immunoliposome fluorescence assay for rapid detection of Escherichia coli O157:H7 in aqueous samples and comparison of the assay with a standard microbiological method.
Appl Environ Microbiol. 2005 Apr;71(4):1856-64. doi: 10.1128/AEM.71.4.1856-1864.2005.
5
Comparison of the sensitivity of manual and automated immunomagnetic separation methods for detection of Shiga toxin-producing Escherichia coli O157:H7 in milk.
J Appl Microbiol. 2002;92(6):1015-20. doi: 10.1046/j.1365-2672.2002.01646.x.
6
Optimizing enrichment conditions for the isolation of Escherichia coli O157 in soils by immunomagnetic separation.
Lett Appl Microbiol. 2002;34(5):365-9. doi: 10.1046/j.1472-765x.2002.01098.x.
7
Ultrasensitive Detection of Escherichia coli O157:H7 by Immunomagnetic Separation and Selective Filtration with Nitroblue Tetrazolium/5-Bromo-4-chloro-3-indolyl Phosphate Signal Amplification.
J Agric Food Chem. 2018 May 16;66(19):4941-4947. doi: 10.1021/acs.jafc.8b00973. Epub 2018 May 4.
8
The optimization of isolation media used in immunomagnetic separation methods for the detection of Escherichia coli O157 in foods.
J Appl Microbiol. 2001 Aug;91(2):373-9. doi: 10.1046/j.1365-2672.2001.01397.x.
9
Rapid detection of Escherichia coli O157:H7 by a fluorescent microsphere-based immunochromatographic assay and immunomagnetic separation.
Anal Biochem. 2019 Jan 1;564-565:32-39. doi: 10.1016/j.ab.2018.10.009. Epub 2018 Oct 11.
10
Recirculating immunomagnetic separation and optimal enrichment conditions for enhanced detection and recovery of low levels of Escherichia coli O157:H7 from fresh leafy produce and surface water.
J Food Prot. 2007 Dec;70(12):2717-24. doi: 10.4315/0362-028x-70.12.2717.
引用本文的文献
1
Development of a robust method for isolation of shiga toxin-positive Escherichia coli (STEC) from fecal, plant, soil and water samples from a leafy greens production region in California.
PLoS One. 2013 Jun 6;8(6):e65716. doi: 10.1371/journal.pone.0065716. Print 2013.
2
Immunomagnetic separation combined with real-time reverse transcriptase PCR assays for detection of norovirus in contaminated food.
Appl Environ Microbiol. 2008 Jul;74(13):4226-30. doi: 10.1128/AEM.00013-08. Epub 2008 Apr 25.
3
Detection of water-borne E. coli O157 using the integrating waveguide biosensor.
Biosens Bioelectron. 2005 Oct 15;21(4):678-83. doi: 10.1016/j.bios.2005.01.005.
4
Deer meat as the source for a sporadic case of Escherichia coli O157:H7 infection, Connecticut.
Emerg Infect Dis. 2002 May;8(5):525-7. doi: 10.3201/eid0805.010373.
5
Development of immunomagnetic separation technique for isolation of Pseudomonas syringae pv. phaseolicola.
Folia Microbiol (Praha). 2000;45(4):321-4. doi: 10.1007/BF02817554.
6
Solid-phase capture of proteins, spores, and bacteria.
Appl Environ Microbiol. 2001 Mar;67(3):1300-7. doi: 10.1128/AEM.67.3.1300-1307.2001.
7
Determination of the sensitivity of a rapid Escherichia coli O157:H7 assay for testing 375-gram composite samples.
Appl Environ Microbiol. 2000 Sep;66(9):4149-51. doi: 10.1128/AEM.66.9.4149-4151.2000.
本文引用的文献
1
Escherichia coli O157:H7: Epidemiology, Pathogenesis, and Methods for Detection in Food.
J Food Prot. 1992 Jul;55(7):555-565. doi: 10.4315/0362-028X-55.7.555.
2
Efficacy of Petrifilm VRB for Enumerating Coliforms and Escherichia coli from Frozen Raw Beef.
J Food Prot. 1987 Dec;50(12):1017-1022. doi: 10.4315/0362-028X-50.12.1017.
3
Isolation of enterohemorrhagic Escherichia coli O157 strains from patients with hemolytic-uremic syndrome by using immunomagnetic separation, DNA-based methods, and direct culture.
J Clin Microbiol. 1996 Mar;34(3):516-9. doi: 10.1128/jcm.34.3.516-519.1996.
4
The isolation and detection of Escherichia coli O157 by use of immunomagnetic separation and immunoassay procedures.
Lett Appl Microbiol. 1996 Mar;22(3):237-43. doi: 10.1111/j.1472-765x.1996.tb01151.x.
5
Immunomagnetic separation as a sensitive method for isolating Escherichia coli O157 from food samples.
Epidemiol Infect. 1994 Aug;113(1):31-9. doi: 10.1017/s0950268800051438.
6
A comparison of immunomagnetic separation and direct culture for the isolation of verocytotoxin-producing Escherichia coli O157 from bovine faeces.
J Med Microbiol. 1994 Jun;40(6):424-7. doi: 10.1099/00222615-40-6-424.
7
Probability of recovering pathogenic Escherichia coli from foods.
Appl Environ Microbiol. 1985 Jun;49(6):1374-8. doi: 10.1128/aem.49.6.1374-1378.1985.
8
Comparison between the adhesion to solid substrata of Streptococcus mitis and that of polystyrene particles.
Appl Environ Microbiol. 1988 Mar;54(3):837-8. doi: 10.1128/aem.54.3.837-838.1988.
9
The epidemiology of infections caused by Escherichia coli O157:H7, other enterohemorrhagic E. coli, and the associated hemolytic uremic syndrome.
Epidemiol Rev. 1991;13:60-98. doi: 10.1093/oxfordjournals.epirev.a036079.
10
Development of the immunomagnetic enrichment method selective for Vibrio parahaemolyticus serotype K and its application to food poisoning study.
Appl Environ Microbiol. 1992 Aug;58(8):2679-82. doi: 10.1128/aem.58.8.2679-2682.1992.
Zotero 插件