Levis C, Giraud T, Dutertre M, Fortini D, Brygoo Y
Station de Pathologie Végétale, INRA, Versailles, France.
FEMS Microbiol Lett. 1997 Dec 15;157(2):267-72. doi: 10.1111/j.1574-6968.1997.tb12783.x.
Telomeric DNA was isolated from the phytopathogenic fungus Botrytis cinerea by PCR using only the oligonucleotide primer (CCCTAA)4. As with other filamentous fungi, B. cinerea has a short TTAGGG telomeric repeat. Telomere-linked restriction fragment length polymorphism (RFLP) was found in strains of B. cinerea isolated from different host plants collected from different regions at different periods. Almost every strain had a specific RFLP pattern, including those collected from the same plant one month apart. Thus, this marker appears to be an excellent tool to show the great polymorphism of B. cinerea strains by fingerprinting. The Southern blots of some strains of B. cinerea showed one band which was much more intense than the others, suggesting that the majority of telomere-associated sequences have the same sequence.
仅使用寡核苷酸引物(CCCTAA)4通过聚合酶链反应(PCR)从植物病原真菌灰葡萄孢中分离出端粒DNA。与其他丝状真菌一样，灰葡萄孢具有短的TTAGGG端粒重复序列。在不同时期从不同地区采集的不同寄主植物中分离得到的灰葡萄孢菌株中发现了端粒连锁限制性片段长度多态性(RFLP)。几乎每个菌株都有特定的RFLP模式，包括那些相隔一个月从同一植物上采集的菌株。因此，这个标记似乎是一个通过指纹图谱显示灰葡萄孢菌株高度多态性的优秀工具。一些灰葡萄孢菌株的Southern杂交显示有一条带比其他带更强，这表明大多数端粒相关序列具有相同的序列。
