Marshall V S, Kalishman J, Thomson J A
Wisconsin Regional Primate Research Center, University of Wisconsin, Madison 53715-1299, USA.
J Med Primatol. 1997 Oct;26(5):241-7. doi: 10.1111/j.1600-0684.1997.tb00218.x.
A technique for nonsurgical embryo transfer in common marmosets was developed. Transfers were either synchronous (ST) or asynchronous (AT). Synchronous transfers (embryo donor and the embryo recipient ovulated on the same day) were performed 5 to 8 days post-ovulation. Asynchronous transfers (embryo donor had ovulated at least 2 days before the embryo recipient) were performed when the recipient was 2 to 4 days post-ovulation (donor was 6 to 8 days post-ovulation). Four pregnancies from nine transfers (44%) were established by AT, and three pregnancies were carried to term. Only 1 of 11 transfers (9%) from ST resulted in a pregnancy, which was lost by Day 40 of gestation. Significantly more infants were born from AT (6 infants from 17 embryos; 35%) than from ST (0 infants from 22 embryos; 0%; p < 0.005). This technique allows experimental analysis of primate postimplantation development and provides a tool for conservation of endangered Callithrichid species.
我们开发了一种普通狨猴非手术胚胎移植技术。移植分为同步移植（ST）和异步移植（AT）。同步移植（胚胎供体和受体在同一天排卵）在排卵后5至8天进行。异步移植（胚胎供体比胚胎受体至少提前2天排卵）在受体排卵后2至4天进行（供体排卵后6至8天）。9次移植中有4次（44%）通过异步移植成功受孕，其中3次妊娠足月。同步移植的11次移植中只有1次（9%）成功受孕，但在妊娠第40天时流产。异步移植出生的婴儿（17个胚胎中有6个婴儿；35%）明显多于同步移植（22个胚胎中无婴儿出生；0%；p<0.005）。该技术能够对灵长类动物植入后发育进行实验分析，并为濒危绢毛猴科物种的保护提供了一种手段。
