Ooshima T, Minami T, Matsumoto M, Fujiwara T, Sobue S, Hamada S
Department of Pedodontics, Osaka University Faculty of Dentistry, Suita, Japan.
Caries Res. 1998;32(1):75-80. doi: 10.1159/000016433.
The cariostatic effect of oolong tea polyphenols administered according to several regimens was examined in specific pathogen-free (SPF) Sprague-Dawley rats given both a diet containing 20% sucrose and infected with S. sobrinus 6715. The crude preparation (OTE) of oolong tea polyphenols showed the most prominent effect on caries reduction in SPF rats when OTE was administered in the drinking water beginning 1 day prior to the inoculation of S. sobrinus 6715, when compared with chromatographically isolated polyphenol fractions (OTF1 and OTF6) of OTE. Reduction in caries development was found even when OTE was given 1 day after inoculation of the organism. OTE was shown to significantly inhibit dental caries in rats at the concentrations of either more than 5 microg/ml in drinking water or more than 10 microg/g in diet. OTF1 and OTF6 also showed significant inhibition of caries induction, with the minimum inhibitory concentration of OTF6 being 50 microg/ml in drinking water and the minimum inhibitory concentration of OTF1 being 100 microg/g in diet. These results indicate that cariostatic activity of OTE was effective even after the establishment of S. sobrinus in the oral cavity and was more effective in drinking water than in diet. Furthermore, OTE may contain some anticaries substances that affect the virulence of S. sobrinus other than glucosyltransferases.
在特定病原体-free（SPF）的斯普拉格-道利大鼠中，研究了按照几种给药方案给予的乌龙茶多酚的防龋效果。这些大鼠食用含20%蔗糖的饮食，并感染了远缘链球菌6715。当从接种远缘链球菌6715前1天开始在饮用水中给予乌龙茶多酚粗提物（OTE）时，与OTE的色谱分离多酚组分（OTF1和OTF6）相比，OTE对SPF大鼠的龋齿减少显示出最显著的效果。即使在接种该微生物1天后给予OTE，也发现龋齿发展有所减少。当饮用水中OTE浓度超过5微克/毫升或饮食中超过10微克/克时，OTE被证明能显著抑制大鼠龋齿。OTF1和OTF6也显示出对龋齿诱导的显著抑制，OTF6在饮用水中的最低抑菌浓度为50微克/毫升，OTF1在饮食中的最低抑菌浓度为100微克/克。这些结果表明，即使在远缘链球菌在口腔中定植后，OTE的防龋活性仍然有效，并且在饮用水中比在饮食中更有效。此外，OTE可能含有一些除葡糖基转移酶外影响远缘链球菌毒力的防龋物质。
