Fullner K J
Department of Microbiology, University of Washington, Seattle 98195-7242, USA.
J Bacteriol. 1998 Jan;180(2):430-4. doi: 10.1128/JB.180.2.430-434.1998.
Nonpolar virB mutants of Agrobacterium tumefaciens were tested for RSF1010 mobilization and extracellular complementation. virB2 to virB11 were essential for transfer in both assays. virB1 was essential only for high frequency transfer of RSF1010 and VirE2. Coordinated transfer of a preassembled T complex is supported by these data and competition studies.
对根癌土壤杆菌的非极性virB突变体进行了RSF1010转移和细胞外互补测试。在这两种测定中,virB2至virB11对于转移至关重要。virB1仅对RSF1010和VirE2的高频转移至关重要。这些数据和竞争研究支持了预组装T复合物的协同转移。