Falchetti M L, Levi A, Molinari P, Verna R, D'Ambrosio E
Dipartimento di Medicina Sperimentale, University of l'Aquila, Rome, Italy.
Nucleic Acids Res. 1998 Feb 1;26(3):862-3. doi: 10.1093/nar/26.3.862.
Telomeric Repeat Amplification Protocol (TRAP) is a sensitive procedure to measure telomerase activity in small samples of cell or tissue extracts. Due to the strict correlation between high levels of telomerase activity and neoplastic transformation, TRAP assay could provide an important diagnostic marker of malignancy. Although the original TRAP assay is very sensitive and some improvements have been described, occasional artifacts still persist in the modified procedures. Here we describe how changes in the sequence of the primer used for the amplification step enhance the reproducibility and sensitivity in the TRAP assay.
端粒重复序列扩增法（TRAP）是一种用于检测细胞或组织提取物小样本中端粒酶活性的灵敏方法。由于端粒酶活性高水平与肿瘤转化之间存在严格的相关性，TRAP 检测可为恶性肿瘤提供重要的诊断标志物。尽管原始的 TRAP 检测非常灵敏，并且已经描述了一些改进方法，但在改进后的程序中偶尔仍会出现假象。在这里，我们描述了用于扩增步骤的引物序列变化如何提高 TRAP 检测的重现性和灵敏度。
