Trebichavský I, Mára M, Sinkora J, Splíchal I, Stĕpánková R
Department of Immunology and Gnotobiology, Academy of Sciences of the Czech Republic, Nový Hrádek, Czech Republic.
Folia Microbiol (Praha). 1997;42(4):403-8. doi: 10.1007/BF02816958.
3-day-old miniature piglets were stimulated in vivo with Bacillus firmus by the intraperitoneal or intragastric route for 1 d. Cells containing IgA and IgG2 were detected in the ileum in all stimulated but not in control animals. The frequency of blood CD3+ cells increased after intraperitoneal administration of B. firmus, the ratio of polymorphonuclears to lymphocytes increased in all stimulated piglets. B. firmus induced antitumor immunity in rats with transplanted Yoshida sarcoma cells. Granular lymphocytes and dead tumor cells were found in peritoneal exudate of stimulated animals. B. firmus induced IFN-gamma synthesis in human blood lymphocytes stimulated in vitro for 1 d. The amount of TNF-alpha produced by these stimulated human peripheral blood mononuclears (PBMC) was lower than that of PBMC stimulated with some other bacterial immunomodulators. Cells containing TGF-beta or IL-8 were not found in human PBMC stimulated with B. firmus.
对3日龄的小型仔猪通过腹腔内或胃内途径用坚强芽孢杆菌进行体内刺激，持续1天。在所有受刺激的仔猪回肠中检测到含有IgA和IgG2的细胞，但在对照动物中未检测到。腹腔注射坚强芽孢杆菌后，血液中CD3 +细胞的频率增加，所有受刺激仔猪的多形核细胞与淋巴细胞的比例增加。坚强芽孢杆菌可诱导移植吉田肉瘤细胞的大鼠产生抗肿瘤免疫力。在受刺激动物的腹腔渗出液中发现了颗粒淋巴细胞和死亡的肿瘤细胞。坚强芽孢杆菌在体外刺激1天的人血淋巴细胞中诱导γ-干扰素合成。这些受刺激的人外周血单核细胞（PBMC）产生的肿瘤坏死因子-α的量低于用其他一些细菌免疫调节剂刺激的PBMC。在用坚强芽孢杆菌刺激的人PBMC中未发现含有转化生长因子-β或白细胞介素-8的细胞。
