Coosemans M, Smits A, Roelants P
Department of Parasitology, Prince Leopold Institute of Tropical Medicine, Antwerp, Belgium.
Am J Trop Med Hyg. 1998 Jan;58(1):70-4. doi: 10.4269/ajtmh.1998.58.70.
A cellulose acetate electrophoresis system was used to study the isozyme polymorphism of the Anopheles gambiae complex in a rural village and a city in southwestern Burkina Faso. In both areas A. gambiae Giles was the dominant species (95%) whereas A. arabiensis Patton represented only 5%. Both species were separated readily by octanol dehydrogenase Odh and mannose phosphate isomerase (Mpi) even if they shared some alleles at these two loci. Polymorphism analysis (13 loci) at the intraspecific level of A. gambiae showed a significant difference between the specimens collected in the city from those collected in the village in their allelic and genotypic frequencies of isocitrate dehydrogenase-1 and malate dehydrogenase-1 and in their allelic frequencies for Mpi. No genetic difference was observed between the human biting A. gambiae collected inside or outside the houses in either the village or the city. The Plasmodium falciparum-infected A. gambiae differed from the noninfected ones in their allelic and genotypic frequencies at Mpi and acid phosphatase (Acp). A two-fold difference in infection rate was found for the genotype Mpi130/130 and Acp110/100 compared with other genotypes. However, infected anophelines were found in all genotypes that belonged to these two enzyme systems. Consequently, no refractory mechanism occurs in these natural populations.
采用醋酸纤维素电泳系统，对布基纳法索西南部一个乡村和一个城市的冈比亚按蚊复合体的同工酶多态性进行了研究。在这两个地区，冈比亚按蚊吉尔斯亚种是优势种（95%），而阿拉伯按蚊帕顿亚种仅占5%。即使这两个物种在这两个位点共享一些等位基因，通过辛醇脱氢酶（Odh）和磷酸甘露糖异构酶（Mpi）也能很容易地将它们区分开来。对冈比亚按蚊种内水平的多态性分析（13个位点）显示，在城市采集的标本与在乡村采集的标本之间，异柠檬酸脱氢酶-1和苹果酸脱氢酶-1的等位基因和基因型频率以及Mpi的等位基因频率存在显著差异。在乡村或城市，在房屋内或房屋外采集的嗜人冈比亚按蚊之间未观察到遗传差异。感染恶性疟原虫的冈比亚按蚊在Mpi和酸性磷酸酶（Acp）的等位基因和基因型频率上与未感染的按蚊不同。与其他基因型相比，基因型Mpi130/130和Acp110/100的感染率存在两倍差异。然而，在属于这两个酶系统的所有基因型中都发现了感染的按蚊。因此，在这些自然种群中不存在抗性机制。
