Smits A, Roelants P, Van Bortel W, Coosemans M
Institute of Tropical Medicine, Laboratory of Medical Entomology, Belgium.
J Med Entomol. 1996 Jul;33(4):545-53. doi: 10.1093/jmedent/33.4.545.
A cellulose acetate electrophoresis system was used to study the Anopheles gambiae complex at 2 villages in Central Imbo Valley and at 1 village in South Imbo Valley (Burundi). In South Imbo, only An. gambiae Giles sensu stricto was present. In the drier Central Imbo, the dominant species was An. arabiensis Patton (97.5%); An. gambiae s.s. represented only 2.5%. Both species were separated readily by Odh and Mpi, because they did not share alleles at those loci. Indoor resting An. gambiae s.s. from South Imbo differed significantly from outdoor resting females at 2 Ioci, Mpi, and Got-2. In Central Imbo, total An. arabiensis did not differ between adjacent villages. Endophagic An. arabiensis significantly differed at loci alpha-Gpd, and Idh-1 between the 2 villages, whereas no difference was observed between exophagic females. Allelic and genotypic frequencies at the locus Mdh-2 were significantly different between indoor and outdoor biting An. arabiensis. Active choice for the best place to bite or to rest seemed to be associated with specific genotypes. The Nei genetic distance values were typical of conspecific populations, ranging from 0.154 to 0.160 between An. gambiae s.s. and An. arabiensis. Even though vector populations were structured at spatial and behavioral levels, they were panmictic, and thus selection of exophilic or exophagic vectors, or both, by insecticide pressure is not likely to occur.
使用醋酸纤维素电泳系统对布隆迪因博谷中部的2个村庄和因博谷南部的1个村庄的冈比亚按蚊复合体进行了研究。在因博谷南部，仅存在冈比亚按蚊狭义种。在较为干旱的因博谷中部，优势种是阿拉伯按蚊（占97.5%）；冈比亚按蚊狭义种仅占2.5%。这两个物种通过Odh和Mpi很容易区分，因为它们在这些位点上不共享等位基因。来自因博谷南部的室内栖息冈比亚按蚊狭义种在Mpi和Got-2这2个位点上与室外栖息雌蚊有显著差异。在因博谷中部，相邻村庄的阿拉伯按蚊总数没有差异。嗜内性阿拉伯按蚊在2个村庄的α-Gpd和Idh-1位点上有显著差异，而嗜外性雌蚊之间没有观察到差异。阿拉伯按蚊室内和室外叮咬群体在Mdh-2位点的等位基因和基因型频率有显著差异。对最佳叮咬或休息地点的主动选择似乎与特定基因型有关。内遗传距离值是同种群体的典型值，冈比亚按蚊狭义种和阿拉伯按蚊之间的范围为0.154至0.160。尽管媒介种群在空间和行为水平上有结构，但它们是随机交配的，因此不太可能因杀虫剂压力而选择嗜外性或嗜外栖性媒介，或两者兼选。
