Hunter D D, Dey R D
Department of Anatomy, West Virginia University School of Medicine, Morgantown 26506-9128, USA.
Neuroscience. 1998 Mar;83(2):591-9. doi: 10.1016/s0306-4522(97)00324-2.
Trigeminal ganglia provides sensory innervation to the rat nasal cavity. The purpose of this study was to identify the location and characterize the neuropeptide content of trigeminal neurons that project specifically to the rat nasal epithelium. The right nasal cavity was instilled with 4 microliters of rhodamine-labelled latex microspheres. Seven, 10 or 14 days after tracer instillation, both trigeminal ganglia were removed and prepared for substance P and calcitonin gene-related peptide immunocyto-chemistry. neurons labelled with microspheres were located in the trigeminal ganglia at the division of the ophthalmic and maxillary nerves and were only found on the side ipsilateral to the instillation. The percentage of labelled cell body profiles in the right trigeminal ganglia averaged 1.61 +/- .04% at seven days. 1.54 +/- .01% at 10 days, and 1.65 +/- .02% at 14 days after instillation. These values were not statistically different, but the fluorescence intensity in labelled neurons was increased after 14 days. The right trigeminal ganglia contained a mean of 166 +/- 13.81 labelled cell body profiles representing 1.60 +/- .09% of the total. Of the labelled profiles, 81.6 +/- 3.27% were immunoreactive for substance P and 35.2 +/- 4.00% for calcitonin gene-related peptide. Sections of nasal mucosa showed that the microspheres were localized only in the epithelial layer and did not enter the lamina propria indicating that the microsphere transport was confined to nerve fibres present in the epithelial layer. These studies have identified specific neurons in the trigeminal ganglia that project to the nasal epithelium. Most of the neurons retrogradely labelled from the nasal cavity contained substance P but some contained calcitonin gene-related peptide, suggesting that the nasal epithelium is predominantly targeted by substance P-containing neurons.
三叉神经节为大鼠鼻腔提供感觉神经支配。本研究的目的是确定特异性投射至大鼠鼻上皮的三叉神经元的位置，并对其神经肽含量进行表征。向右侧鼻腔滴注4微升罗丹明标记的乳胶微球。在滴注示踪剂7天、10天或14天后，取出双侧三叉神经节，准备进行P物质和降钙素基因相关肽免疫细胞化学检测。用微球标记的神经元位于三叉神经节内眼神经和上颌神经分支处，且仅在滴注侧的同侧发现。右侧三叉神经节中标记细胞体轮廓的百分比在滴注后7天平均为1.61±0.04%，10天为1.54±0.01%，14天为1.65±0.02%。这些值在统计学上无差异，但标记神经元的荧光强度在14天后增加。右侧三叉神经节平均含有166±13.81个标记细胞体轮廓，占总数的1.60±0.09%。在标记轮廓中，81.6±3.27%对P物质免疫反应阳性，35.2±4.00%对降钙素基因相关肽免疫反应阳性。鼻黏膜切片显示微球仅定位在上皮层，未进入固有层，这表明微球运输仅限于上皮层中的神经纤维。这些研究确定了三叉神经节中投射至鼻上皮的特定神经元。大多数从鼻腔逆行标记的神经元含有P物质，但有些含有降钙素基因相关肽，这表明鼻上皮主要由含P物质的神经元靶向。
